Down regulation of interleukin-1beta-induced nitric oxide production in lacrimal gland acinar cells by sex steroids

Current Eye Research
Clay Beauregard, Paul Brandt

Abstract

Because the ocular surface is constantly exposed to allergens and irritants, it was reasoned that one cause of dry eye might be damage from inflammatory responses normally regulated by sex steroids. To test this hypothesis, we determined if sex steroids could down regulate nitric oxide (NO) production induced by interleukin-1beta (IL-1beta) in cultured rabbit lacrimal gland acinar cells. Cultured rabbit lacrimal gland acinar cells were exposed to IL-1beta to stimulate NO production. Stimulated cells were treated with different sex steroids and expression of iNOS protein determined by Western blotting and NO production by a nitrate/nitrite colorimetric assay. It was found that the androgens testosterone, dihydrotestosterone, dehydroepiandrosterone and dehydroepiandrosterone-sulfate and 17beta-estradiol were able to inhibit interleukin-1beta-induced NO production in rabbit lacrimal gland acinar cells at physiological concentrations, while progesterone was not able to inhibit NO production. Sex steroid inhibition of NO production was not due to down regulation of iNOS protein production nor was it due to down regulation of GTP cyclohydrolase I with consequent loss of tetrahydrobiopterin production. The results reported here show t...Continue Reading

References

Nov 1, 1995·American Journal of Obstetrics and Gynecology·J P Figueroa, G A Massmann
Sep 27, 1996·The Journal of Biological Chemistry·J J PalvimoO A Jänne
Feb 1, 1997·Endocrine·A M AzzaroloD W Warren
Dec 28, 1999·The British Journal of Ophthalmology·E M RochaD A Sullivan
May 4, 2000·Acta Ophthalmologica Scandinavica·L A WickhamD A Sullivan
Jan 12, 2001·Physiological Reviews·E E Strehler, D A Zacharias
Nov 22, 2001·JAMA : the Journal of the American Medical Association·D A SchaumbergM R Dana

❮ Previous
Next ❯

Citations

Aug 12, 2009·Clinical Ophthalmology·Koray Gumus, Dwight H Cavanagh
Jan 16, 2014·The Journal of Endocrinology·Donato CalabreseRoberto Cosimo Melcangi
Oct 8, 2014·Current Eye Research·Piera VersuraEmilio C Campos
Jan 8, 2014·Experimental Eye Research·Alexey S AndradeMara S Benfato
Jul 1, 2017·International Journal of Molecular Sciences·Xiaobo ZhangWei Li
Jul 21, 2007·Endocrine Reviews·Rainer H Straub
Mar 24, 2020·The World Journal of Men's Health·Abdulmaged M Traish
Dec 29, 2020·Acta histochemica·Kristina ŠemanjskiDavor Ježek

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

American Journal of Contact Dermatitis : Official Journal of the American Contact Dermatitis Society
E B Desciak, J G Marks
American Journal of Contact Dermatitis : Official Journal of the American Contact Dermatitis Society
Amanda E Tauscher, Donald V Belsito
American Journal of Contact Dermatitis : Official Journal of the American Contact Dermatitis Society
M R HollidayI Kimber
Immunology and Allergy Clinics of North America
Marc Antezana, Frank Parker
© 2021 Meta ULC. All rights reserved