Abstract
Osteoblast Ca2+ channels play a fundamental role in controlling intracellular and systemic Ca2+ homeostasis. A reverse transcription-polymerase chain reaction strategy was used to determine the molecular identity of voltage-sensitive calcium channels present in ROS 17/2.8 osteosarcoma cells. The amino acid sequences encoded by the two resultant PCR products matched the alpha1C-a and the alpha1C-d isoforms. The ability of 1, 25-dihydroxyvitamin D3 (1,25(OH)2D3) and structural analogs to modulate expression of voltage-sensitive calcium channel mRNA transcripts was then investigated. ROS 17/2.8 cells were cultured for 48 h in the presence of either 1,25(OH)2D3,1,24-dihydroxy-22-ene-24-cyclopropyl D3 (analog BT) or 25-hydroxy-16-ene-23-yne-D3 (analog AT), and the levels of mRNA encoding alpha1C were quantitated using a competitive reverse transcription-polymerase chain reaction assay. We found that 1, 25(OH)2D3 and analog BT reduced steady state levels of alpha1C mRNA. Conversely, the Ca2+-mobilizing analog AT did not alter steady state levels of voltage-sensitive calcium channel mRNA. Since analog BT, but not analog AT, binds and transcriptionally activates the nuclear receptor for 1,25(OH)2D3, these findings suggest that the down...Continue Reading
References
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A S YuJ Lytton
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S SeinoG I Bell
Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·A W NormanM C Farach-Carson
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·N M Soldatov
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R BortellG S Stein
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·N KawaguchiM Noda
Aug 15, 1991·European Journal of Biochemistry·M BielF Hofmann
Oct 1, 1991·Endocrinology·M C Farach-CarsonA W Norman
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T P SnutchN Davidson
Apr 4, 1991·Nature·Y MoriT Furuichi
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M NodaD T Denhardt
May 1, 1987·Science·J M CaffreyM D Schneider
Oct 7, 1988·Science·W A Catterall
Jul 20, 1989·Nature·A MikamiS Numa
Jul 20, 1989·Nature·E Perez-ReyesL Birnbaumer
Dec 1, 1989·Archives of Biochemistry and Biophysics·E Kyeyune-NyombiD D Strong
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S A KernerJ W Pike
Jan 1, 1986·Annual Review of Nutrition·M R Haussler
Dec 1, 1986·Journal of Steroid Biochemistry·Y Yoshimoto, A W Norman
Jul 23, 1987·Nature·T TanabeS Numa
Jan 1, 1981·Proceedings of the Society for Experimental Biology and Medicine·J E PuzasD J Baylink
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·E L BarryP A Friedman
Jul 1, 1993·Diabetes·Y IwashimaK S Polonsky
Nov 1, 1994·Nutrition Reviews·S S Hannah, A W Norman
Mar 24, 1995·The Journal of Biological Chemistry·S J SlaterC D Stubbs
Dec 1, 1994·Endocrinology·R KhouryM C Farach-Carson
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·R L DuncanK A Hruska
Jan 1, 1996·Connective Tissue Research·J G MeszarosM C Farach-Carson
Citations
Mar 29, 2000·Journal of Cellular Physiology·M A Said AhmedA J El Haj
Feb 19, 1999·Gene·D ZoccolaD Allemand
Apr 15, 2008·Trends in Endocrinology and Metabolism : TEM·A RevelliJ Tesarik
Aug 16, 2003·Bone·Laura P Zanello, Anthony W Norman
Jan 12, 1999·The Biochemical Journal·G J Barritt
Feb 23, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J C LozaR Dziak
Jul 14, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·X T WangS E Guggino
Nov 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I MathovT Bellido
Jun 13, 2001·Biochemical and Biophysical Research Communications·Y GuS J Publicover
Oct 23, 2010·The International Journal of Neuroscience·Mohsen TaghizadehSayyed Alireza Talaei Zavareh
Apr 23, 2015·PloS One·Xuemei ZhangHui Cai
Sep 9, 2004·American Journal of Physiology. Renal Physiology·Edna EfratiIsrael Zelikovic
Oct 14, 2005·American Journal of Physiology. Cell Physiology·Joel J BerghMary C Farach-Carson
Mar 21, 2008·American Journal of Physiology. Cell Physiology·Annamaria MorelliMario Maggi
Mar 18, 2000·The Journal of Biological Chemistry·R LiuM C Farach-Carson
Feb 1, 2000·The Journal of Biological Chemistry·A MiyauchiT Fujita
Aug 2, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ying ShaoMary C Farach-Carson
Feb 9, 2000·American Journal of Physiology. Endocrinology and Metabolism·S PuriR R MacGregor
Mar 6, 2009·American Journal of Physiology. Cell Physiology·Ying ShaoMary C Farach-Carson
Oct 28, 2020·Annual Review of Physiology·Geoffrey S PittChike Cao
Mar 16, 2021·Current Osteoporosis Reports·Christian S WrightWilliam R Thompson
Jun 9, 2001·Cell Calcium·T HattoriM Naito
Jun 20, 2006·Cell Calcium·Lawrence D BrewerOlivier Thibault
Mar 9, 2006·Nano Letters·Laura P ZanelloRobert C Haddon