Down-regulation of lncRNA MEG3 promotes endothelial differentiation of bone marrow derived mesenchymal stem cells in repairing erectile dysfunction

Life Sciences
Xiang SunDong-Shui Li

Abstract

In the treatment of diabetes mellitus associated erectile dysfunction (DMED), the intracavernous and periprostatic implantations of bone marrow derived mesenchymal stem cells (BM-MSCs) represent the new therapeutic approaches with great applied prospect. However, the specific mechanisms of BM-MSCs protecting erectile function remain largely unknown. The DMED rats were induced and the erectile function was assessed in the models with or without BM-MSCs implantation using intracavernous pressure (ICP)/mean arterial pressure (MAP) ratio. The differentiation of BM-MSCs toward endothelial cells (ECs) was induced by exogenous vascular endothelial growth factor (VEGF) in vitro. RNA pull-down and RIP assays were performed to explore the interaction between MEG3 and FOXM1 protein. Intracavernous implantation of BM-MSCs effectively improved the erectile function of DMED rats, which was accompanied by a significant decrease in the expression of MEG3 in the corpus cavernosum tissues. Also, our study revealed that MEG3 expression was significantly down-regulated during the endothelial differentiation of BM-MSCs in vitro. The down-regulation of MEG3 was further confirmed to be conducive to the differentiation of BM-MSCs toward ECs. More impo...Continue Reading

Citations

May 23, 2020·Andrologia·Sixiang ChenKeyang Xu
Apr 6, 2019·World Journal of Stem Cells·Zhong-Yu XieHui-Yong Shen
Mar 28, 2020·Stem Cells International·Selma MaachaChiara Cugno
Dec 15, 2020·Briefings in Bioinformatics·Elham KazemiKawsar Ahmed
May 28, 2021·BMC Bioinformatics·Swapna Vidhur DaulatabadSarath Chandra Janga
Jun 3, 2021·International Journal of Molecular Sciences·Pei-Fang HsiehPei-Ling Hsieh
Aug 10, 2021·Frontiers in Cell and Developmental Biology·Yanlei YangRobert Chunhua Zhao
Aug 25, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardAfshin Taheriazam

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