Down-regulation of long non-coding RNA AFAP1-AS1 inhibits tumor growth, promotes apoptosis and decreases metastasis in thyroid cancer

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Wencheng DaiWeixian Chen

Abstract

Long non-coding RNAs (lncRNAs), a new type of transcripts, play important roles in various cellular biological processes, involving tumorigenesis. Previous studies showed that lncRNA AFAP1-AS1 was aberrantly expressed in numerous cancers. Nevertheless, we know quite a little about the expression pattern and biological function of AFAP1-AS1 in thyroid cancer. In this study, we adopted the quantitative real-time PCR (qRT-PCR) to detect the expression of AFAP1-AS1 in thyroid cancer tissues. We discovered that expression of AFAP1-AS1 was increased in thyroid cancer tissues. MTT assays elucidated that down-regulation of AFAP1-AS1 could suppress growth of thyroid cancer cells. And the results of flow cytometry analysis indicated knockdown of AFAP1-AS1 induced apoptosis in thyroid cancer. Transwell assay was applied to show decreased cell migration in thyroid cancer as a result of down-regulation of AFAP1-AS1. Hence, our study provided evidence for our hypothesis that AFAP1-AS1 could be a therapeutic target for thyroid cancer.

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Citations

Feb 26, 2019·Oncology Research and Treatment·Mohammad-Reza Mahmoudian-SaniGholam-Reza Mobini
Feb 5, 2019·Journal of Cellular Physiology·Lei Yue, Jing Guo
Jan 4, 2020·Oncology Letters·Jin-Zhu LuoLing-Jie Zhang
Mar 24, 2020·Endocrine Reviews·Vinaya Srirangam Nadhamuni, Márta Korbonits
Nov 11, 2019·Experimental and Molecular Pathology·Soudeh Ghafouri-FardMohammad Taheri

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