Down-regulation of long non-coding RNA HOTAIR inhibits invasion and migration of oesophageal cancer cells via up-regulation of microRNA-204

Journal of Cellular and Molecular Medicine
Ai-Hua WangLu-Ning Li

Abstract

Oesophageal cancer is a progressive tumour with high mortality. However, therapies aimed at treating oesophageal cancer remain relatively limited. Accumulating studies have highlighted long non-coding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR), microRNA-204 (miR-204) and homeobox C8 (HOXC8) in the progression of oesophageal cancer. Herein, we tried to demonstrate the function of HOTAIR, miR-204 and HOXC8 in oesophageal cancer and their relationship. Differentially expressed genes involved in oesophageal cancer were identified. The endogenous expression of HOTAIR and miR-204 in oesophageal cancer cell lines was altered to elucidate their effects and to identify the interaction among HOTAIR, miR-204 and HOXC8. We also explored the underlying regulatory mechanisms of HOTAIR and miR-204 with siRNA against HOTAIR, miR-204 mimic or miR-204 inhibitor. Cell proliferation, migration, invasion and apoptosis were subsequently detected. Xenograft in nude mice was induced to evaluate tumourigenicity. miR-204 was down-regulated, while HOTAIR and HOXC8 were up-regulated in the oesophageal cancer tissues. HOTAIR could competitively bind to miR-204 and miR-204 could further target HOXC8. The oesophageal cancer cells treated with si-HOTA...Continue Reading

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Citations

Aug 18, 2020·Cancer Management and Research·Linlin GuoLili Liu
Aug 14, 2020·Journal of Clinical Laboratory Analysis·Chenhui Bao, Lin Guo
Mar 17, 2020·Cancer Biomarkers : Section a of Disease Markers·Huixia Li, Congjin Guan
Jan 20, 2021·Cellular and Molecular Life Sciences : CMLS·Siyuan LuanYong Yuan
Dec 31, 2020·Cancers·Ana PaçoRenata Freitas
Jul 24, 2020·Anti-cancer Agents in Medicinal Chemistry·Onur TokgunHakan Akca

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Methods Mentioned

BETA
surgical resection
transfection
PCR
electrophoresis
xenografts
RIP
flow cytometry
xenograft

Software Mentioned

SPSS
R package “ edgeR
Image
Pro Plus Analysis
IPP
Proplus
multitest

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