Down-regulation of metallothionein, a reactive oxygen scavenger, by the small GTPase OsRac1 in rice

Plant Physiology
Hann Ling WongKo Shimamoto

Abstract

Metallothioneins are small, ubiquitous Cys-rich proteins known to be involved in reactive oxygen species (ROS) scavenging and metal homeostasis. We found that the expression of a metallothionein gene (OsMT2b) was synergically down-regulated by OsRac1 and rice (Oryza sativa) blast-derived elicitors. Transgenic plants overexpressing OsMT2b showed increased susceptibility to bacterial blight and blast fungus. OsMT2b-overexpressing cells showed reduced elicitor-induced hydrogen peroxide production. In contrast, homozygous OsMT2b::Tos17-inserted mutant and OsMT2b-RNAi-silenced transgenic cells showed significantly higher elicitor-induced hydrogen peroxide production than the wild-type cells. In vitro assay showed that recombinant OsMT2b protein possessed superoxide- and hydroxyl radical-scavenging activities. Taken together, these results showed that OsMT2b is an ROS scavenger and its expression is down-regulated by OsRac1, thus potentiating ROS, which function as signals in resistance response. The results suggest that OsRac1 plays a dual role as an inducer of ROS production and a suppressor of ROS scavenging.

References

May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·M A SchwarzJ R Lancaster
Oct 1, 1993·The Biochemical Journal·N J RobinsonP J Jackson
Apr 1, 1993·The Biochemical Journal·L S Chubatsu, R Meneghini
Mar 1, 1993·Free Radical Biology & Medicine·M Sato, I Bremner
Mar 1, 1996·Current Biology : CB·W ChiuJ Sheen
Oct 1, 1996·The Plant Cell·K E Hammond-Kosack, J D Jones
Nov 1, 1996·Plant Molecular Biology·H M HsiehP C Huang
Mar 1, 1997·The Plant Journal : for Cell and Molecular Biology·T Aoyama, N H Chua
May 1, 1997·The Plant Journal : for Cell and Molecular Biology·H TakahashiD F Klessig
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·S ChamnongpolW Van Camp
Apr 17, 1999·The Plant Journal : for Cell and Molecular Biology·A TakahashiK Shimamoto
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·T KawasakiK Shimamoto
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·R MittlerB E Ellis
Mar 4, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·A V Peskin, C C Winterbourn
Jan 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·E OnoK Shimamoto
Feb 24, 2001·Plant Molecular Biology·M C Heath
Jul 19, 2001·Genes & Development·E LemichezN H Chua
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M DelledonneC Lamb
Nov 10, 2001·Science·I R Vetter, A Wittinghofer
May 23, 2002·Cellular and Molecular Life Sciences : CMLS·P CoyleA M Rofe
Jun 5, 2002·The Plant Cell·Zhenbiao Yang
Jun 18, 2002·Science·Airica Baxter-BurrellJulia Bailey-Serres
Aug 3, 2002·Plant & Cell Physiology·Kenji UmemuraHideki Usami
Sep 12, 2002·Annual Review of Plant Biology·Christopher Cobbett, Peter Goldsbrough
Sep 18, 2002·Trends in Plant Science·Ron Mittler
Sep 19, 2002·Proceedings of the National Academy of Sciences of the United States of America·Utut SuharsonoKo Shimamoto
Sep 28, 2002·Blood·Gary M Bokoch, Becky A Diebold
Apr 18, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ryuya ShimodaMichael P Waalkes
Jul 2, 2003·FEBS Letters·Ganesh K AgrawalRandeep Rakwal

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Citations

Jan 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Komudi SinghHay-Oak Park
Mar 23, 2012·Plant & Cell Physiology·Sung-Hyun KimKo Shimamoto
Oct 9, 2007·Molecular Plant-microbe Interactions : MPMI·Mariana ObertelloClaudine Franche
Mar 29, 2013·Open Biology·Daisuke UranoAlan M Jones
Aug 7, 2009·Plant Physiology·Bianka Steffens, Margret Sauter
May 15, 2012·Plant Physiology·Josefine Nymark HegelundJan Kofod Schjoerring
Aug 30, 2008·The Plant Cell·Ayako NakashimaKo Shimamoto

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