Down-regulation of miR-206 is associated with Hirschsprung disease and suppresses cell migration and proliferation in cell models

Scientific Reports
Ankur SharanYankai Xia

Abstract

Hirschsprung disease (HSCR) is a well-known congenital digestive disease that originates due to the developmental disorder of neural crest cells. MiR-206 is kown to have a relationship with digestive malfunctions. Therefore, we investigated whether or not miR-206 was involved in the pathogenesis of HSCR. qRT-PCR and Western blot assays were used to detect the expression levels of miRNA and mRNAs, and proteins in case and control tissue samples and two cell lines (293T and SH-SY5Y). The functions of miR-206 in vitro were measured by transwell assay, CCK8 assay and flow cytometry. Finally, we conducted dual-luciferase reporter assay to verify the connections between miR-206 and the target mRNA SDPR. Down-regulation of miR-206 was found in HSCR case tissue samples compared with controls, which was validated to be connected with the increased level of mRNA and protein of SDPR by qRT-PCR and dual-luciferase reporter assay. Moreover, miR-206 suppressed the cell migration and proliferation and silencing of SDPR could rescue the extent of the suppressing effects by miR-206 inhibitor. The findings suggest that miR-206 may play a significant role in the pathogenesis of HSCR, as well as inhibiting the cell migration and proliferation by t...Continue Reading

References

Nov 6, 2001·Journal of Medical Genetics·J Amiel, S Lyonnet
Mar 23, 2004·Nature Medicine·Lisette AcevedoWilliam C Sessa
Apr 15, 2005·Nature·Eileen Sproat EmisonAravinda Chakravarti
Jan 16, 2009·Nature Reviews. Molecular Cell Biology·Peter Brodersen, Olivier Voinnet
Jun 16, 2009·Nature Cell Biology·Carsten G HansenBenjamin J Nichols
Jun 11, 2010·Annual Review of Biochemistry·Marc Robert FabianWitold Filipowicz
Apr 18, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Rui WangXu Ma
Jan 24, 2013·PloS One·Raquel María FernándezSalud Borrego
Apr 13, 2013·Pediatric Surgery International·Weibing TangYankai Xia
Jun 27, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·Sonja J McKeownHeather M Young
Jul 28, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Yun-Ping BaoHua-Song Luo
Oct 26, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Xiuli LiuQiang Wang
Jan 21, 2014·Biochemical and Biophysical Research Communications·Yihui WangXishan Wang

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Citations

Jan 26, 2016·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·J M Rogers
Nov 18, 2015·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Z ShenW Tang
Nov 3, 2016·Pediatric Research·Consolato Maria SergiDavid D Eisenstat
Jun 30, 2019·International Journal of Molecular Sciences·Ana TorroglosaSalud Borrego
Jan 9, 2019·Orphanet Journal of Rare Diseases· GunadiAkhmad Makhmudi
Oct 27, 2020·The Journal of International Medical Research·Yi ZhengWei Zhong

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Methods Mentioned

BETA
PCR
transfection
Flow Cytometry
Assay

Software Mentioned

pro Plus
Stata
Pictar
Image J
Targetscan
Graphpad
DIANA LAB
FlowJo
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