Down-regulation of molecular chaperone 78-kd glucose-regulated protein/immunoglobulin-binding protein expression involved in enhancement of human RS cell mutability

Pancreas
Junko HiranoNobuo Suzuki

Abstract

Enhancement of cell mutability via extracellular materials of cancer cells is a crucial event leading to the development of cancers; however, the activation process of mutability is still not well understood. In this study, to identify the regulatory mechanism of cell mutability, we investigated mutability modulated in response to human pancreatic cancer cell-conditioned medium and identified the candidates for cellular molecules involved in the mutability modulation. To test the mutation-modulating effects of the conditioned medium, human RS cells were cultured with medium derived by culturing human pancreatic cancer KP-4 cells, followed by irradiation with UV (mainly 254 nm in wavelength). Mutations were detected by phenotypic ouabain resistance and genetic base substitution of K-ras codon 12. Messenger RNA differential display was used to identify genes that were differentially expressed between conditioned medium-treated and mock-treated RSa cells. The influence of 78-kd glucose-regulated protein/immunoglobulin-binding protein (GRP78/BiP) expression on mutability was assessed by the down-regulation of GRP78/BiP using antisense oligonucleotides or antisense complementary DNA. The UV-induced mutagenicity in RS cells was stren...Continue Reading

References

May 1, 1976·Journal of the National Cancer Institute·T KuwataN Morinaga
Jan 1, 1992·DNA Sequence : the Journal of DNA Sequencing and Mapping·S K Wooden, A S Lee
Nov 1, 1988·Mutation Research·N Suzuki, H Suzuki
Apr 1, 1985·Mutation Research·H SuzukiK Hiraga
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J J SciandraC S Hughes
Nov 22, 1995·Biochemical and Biophysical Research Communications·T Hiwasa, E Kominami
Jan 1, 1994·Critical Reviews in Eukaryotic Gene Expression·E LittleA S Lee
Nov 10, 1998·International Journal of Cancer. Journal International Du Cancer·N SuzukiY Hayashi
Apr 10, 1999·Genes & Development·W L de LaatJ H Hoeijmakers
Jun 26, 1999·The European Journal of Neuroscience·Y NakanoM Takeda
Feb 16, 2000·Gene·D P Batty, R D Wood
Jun 25, 2002·Best Practice & Research. Clinical Gastroenterology·Roland M Schmid
Feb 18, 2004·World Journal of Gastroenterology : WJG·De-Qing MuQiao-Jian Xu
Mar 31, 2004·Lancet·Donghui LiJames L Abbruzzese
Sep 20, 2005·Biochimica Et Biophysica Acta·Therese Deramaudt, Anil K Rustgi
Mar 15, 2006·Annals of the New York Academy of Sciences·Johnny KaoStephen J Kron

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.