Down regulation of multidrug resistance protein-1 expression in patients with early rheumatoid arthritis exposed to methotrexate as a first disease-modifying antirheumatic drug

Annals of the Rheumatic Diseases
S L HiderI N Bruce

Abstract

Methotrexate (MTX) is the current gold standard conventional disease-modifying antirheumatic drug (DMARD) and is effluxed from cells by several transmembrane proteins, including multidrug resistance protein-1 (MRP1). It is hypothesised that the overexpression of these proteins may mediate reduced efficacy of MTX. To date, it is unclear how expression of these proteins changes over time or after exposure to drugs. To compare MRP1 expression in newly diagnosed patients with DMARD-naive rheumatoid arthritis with that in healthy controls and to investigate how MRP1 expression changed after exposure to MTX. 18 newly diagnosed patients with DMARD-naive rheumatoid arthritis and 14 healthy controls were recruited. Peripheral blood mononuclear cell counts were taken at baseline and after 6 months' treatment with MTX. Cells were separated by density gradient centrifugation and MRP1 expression was measured using the QCRL-1 monoclonal antibody. MRP1 expression in patients did not seem to be up regulated compared with that in healthy controls. In patients who were positive for MRP1 at baseline (61%), treatment with MTX and folic acid led to a marked down regulation of MRP1 expression at 6 months. In patients with rheumatoid arthritis expres...Continue Reading

Citations

Feb 9, 2010·Pharmacogenomics·Wouter M KooloosHenk-Jan Guchelaar
Mar 25, 2014·International Journal of Molecular Sciences·Andrea Picchianti-DiamantiRaffaele D'Amelio
Mar 25, 2015·Expert Opinion on Drug Metabolism & Toxicology·Anna Klukovits, Peter Krajcsi
Dec 31, 2015·Diagnostic Pathology·Tamás MicsikIstván Peták
Jul 17, 2013·The Journal of Rheumatology·Lisa K StampPaul A Hessian
Aug 22, 2020·Translational Vision Science & Technology·Ravichandra TagirasaSoumyava Basu
Jul 18, 2021·Colloids and Surfaces. B, Biointerfaces·Tais Monteiro MagneRalph Santos-Oliveira
Jul 20, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yi-Jin WuWei Wei

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