Down-regulation of MutS homolog 3 by hypoxia in human colorectal cancer.

Biochimica Et Biophysica Acta
Jie LiHiromichi Hemmi

Abstract

Down-regulation of hMSH3 is associated with elevated microsatellite alterations at selected tetranucleotide repeats and low levels of microsatellite instability in colorectal cancer (CRC). However, the mechanism that down-regulates hMSH3 in CRC is not known. In this study, a significant association between over-expression of glucose transporter 1, a marker for hypoxia, and down-regulation of hMSH3 in CRC tissues was observed. Therefore, we examined the effect of hypoxia on the expression of hMSH3 in human cell lines. When cells with wild type p53 (wt-p53) were exposed to hypoxia, rapid down-regulation of both hMSH2 and hMSH3 occurred. In contrast, when null or mutated p53 (null/mut-p53) cells were exposed to hypoxia, only hMSH3 was down-regulated, and at slower rate than wt-p53 cells. Using a reporter assay, we found that disruption of the two putative hypoxia response elements (HREs) located within the promoter region of the hMSH3 abrogated the suppressive effect of hypoxia on reporter activity regardless of p53 status. In an EMSA, two different forms of HIF-1α complexes that specifically bind to these HREs were detected. A larger complex containing HIF-1α predominantly bound to the HREs in hypoxic null/mut-p53 cells whereas a...Continue Reading

References

Jan 9, 2001·International Journal of Cancer. Journal International Du Cancer·L XuJ Jen
Mar 27, 2003·Gene·Michitsune AritaHiroyuki Shimatake
Dec 24, 2003·The Tohoku Journal of Experimental Medicine·Hisahiko SugiyamaHiromichi Hemmi
Feb 26, 2004·Journal of Vascular Surgery·Jeffrey A KalishFrank W Logerfo
Jul 29, 2004·DNA Repair·Lovorka StojicJosef Jiricny
Sep 24, 2004·International Journal of Cancer. Journal International Du Cancer·Gad SingerHolger Moch
Apr 1, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Maija R J Kohonen-CorishE Leslie Bokey
Jun 15, 2005·Annual Review of Biochemistry·Thomas A Kunkel, Dorothy A Erie
Aug 23, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Maximilian BurgerRobert Stoehr
Sep 22, 2007·Bulletin of Mathematical Biology·K E Thompson, J A Royds
Apr 4, 2008·Cancer Letters·Xiao-Feng Li, Joseph A O'Donoghue
May 31, 2008·Stem Cells·Francisco Javier Rodríguez-JiménezJosé-María Sánchez-Puelles
Oct 17, 2008·Cancer Research·Astrid C HaugenMinoru Koi
Jul 22, 2009·BMC Cancer·Fu-Yen ChungShiu-Ru Lin
Aug 18, 2009·Developmental Biology·Tamar Juven-Gershon, James T Kadonaga
Dec 1, 2010·Asia-Pacific Journal of Clinical Oncology·Barry IacopettaBenhur Amanuel
Jan 29, 2011·The Journal of Obstetrics and Gynaecology Research·Minoru Koi, Clement R Boland

❮ Previous
Next ❯

Citations

Oct 25, 2013·Genome Integrity·Kaisa R LuotoRobert G Bristow
Jan 17, 2020·International Journal of Molecular Sciences·Michitsune AritaHiromichi Hemmi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.