Down-regulation of NR4A1 in follicular thyroid carcinomas is restored following lithium treatment.

Clinical Endocrinology
Cléber P CamachoJanete M Cerutti

Abstract

The identification of follicular thyroid adenoma-associated transcripts will lead to a better understanding of the events involved in pathogenesis and progression of follicular tumours. Using Serial Analysis of Gene Expression, we identified five genes that are absent in a malignant follicular thyroid carcinoma (FTC) library, but expressed in follicular adenoma (FTA) and normal thyroid libraries. NR4A1, one of the five genes, was validated in a set of 27 normal thyroid tissues, 10 FTAs and 14 FTCs and three thyroid carcinoma cell lines by real time PCR. NR4A1 can be transiently increased by a variety of stimuli, including lithium, which is used as adjuvant therapy of thyroid carcinoma with (131)I. We tested if lithium could restore NR4A1 expression. The expression of other genes potentially involved in the same signalling pathway was tested. To this end, lithium was used at different concentration (10 mm or 20 mm) and time (2 h and 24 h) and the level of expression was tested by quantitative PCR. We next tested if Lithium could affect cell growth and apoptosis. We observed that NR4A1 expression was under-expressed in most of the FTCs investigated, compared with expression in normal thyroid tissues and FTAs. We also found a posi...Continue Reading

References

Jan 1, 1989·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·B EstourH Klandorf
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T G HazelL F Lau
Oct 1, 1972·The Journal of Clinical Investigation·R TempleJ Wolff
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·F WeihR Bravo
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y PekarskyC M Croce
Jun 28, 2001·Physiological Reviews·A Aranda, A Pascual
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y HuangA de la Chapelle
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kathy BoonGregory J Riggins
Aug 10, 2002·The American Journal of Surgical Pathology·Marina N NikiforovaYuri E Nikiforov
Jan 10, 2003·The Journal of Clinical Endocrinology and Metabolism·Linda CheungBruce G Robinson
Jan 30, 2003·Molecular Endocrinology·Guanjun ChengJudy L Meinkoth
Feb 1, 2003·Molecular Genetics and Metabolism·Julia Penso, Rivka Beitner
May 2, 2003·European Journal of Endocrinology·Efisio PuxedduFausto Santeusanio
May 3, 2003·The Journal of Clinical Endocrinology and Metabolism·Marina N NikiforovaYuri E Nikiforov
Jun 5, 2003·Biochimica Et Biophysica Acta·Rachel H GilesHans Clevers
Aug 22, 2003·Thyroid : Official Journal of the American Thyroid Association·Serik MeirmanovIchiro Sekine
Sep 4, 2003·Journal of Endocrinological Investigation·J M CeruttiE T Kimura
Apr 16, 2004·The Journal of Clinical Investigation·Janete M CeruttiGregory J Riggins
Jul 27, 2004·Clinical Endocrinology·Cláudia C D NakabashiRui M B Maciel
Mar 24, 2005·Thyroid : Official Journal of the American Thyroid Association·L A T ArnaldiJ M Cerutti
May 6, 2005·The Journal of Biological Chemistry·Sudhakar ChintharlapalliStephen Safe
Jan 20, 2006·Thyroid : Official Journal of the American Thyroid Association·David S CooperUNKNOWN American Thyroid Association Guidelines Taskforce
Apr 11, 2006·Nuclear Receptor Signaling·Megan A Maxwell, George E O Muscat
May 11, 2006·JAMA : the Journal of the American Medical Association·Louise Davies, H Gilbert Welch
May 27, 2006·European Journal of Endocrinology·Furio PaciniUNKNOWN European Thyroid Cancer Taskforce
Jun 3, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Janete M CeruttiGregory J Riggins
Sep 21, 1968·British Medical Journal·M SchouT Olsen
Nov 15, 2006·Cell Biology and Toxicology·D KarlovicM Osmak
May 23, 2007·Nature Medicine·Shannon E MullicanOrla M Conneely
Apr 24, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yasushi FujiiShigeo Koyasu

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Citations

Nov 26, 2009·Molecular and Cellular Endocrinology·Beatriz C G Freitas, Janete M Cerutti
Jun 3, 2016·Breast Cancer Research and Treatment·Joseph GeradtsGenglin Jin
Nov 22, 2019·Frontiers in Oncology·Shilpa ThakurJoanna Klubo-Gwiezdzinska
May 9, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Helen M MohanEvelyn P Murphy
Jun 15, 2010·Free Radical Biology & Medicine·Maria Sharmila A SousaJanete M Cerutti

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