Down-regulation of secreted lymphocyte antigen-6/urokinase-type plasminogen activator receptor-related peptide-1 (SLURP-1), an endogenous allosteric alpha7 nicotinic acetylcholine receptor modulator, in murine and human asthmatic conditions

Biochemical and Biophysical Research Communications
Osamu NarumotoNaomi Yamashita

Abstract

Whereas acetylcholine (ACh) acts as a bronchoconstrictor and stimulator of mucus secretion from bronchial epithelium, it acts via alpha7 nicotinic Ach receptors (nAChRs) on macrophages in the airways to exert anti-inflammatory effects by reducing synthesis of pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha). Moreover, the effects of ACh are modified by secreted ly-6/urokinase-type plasminogen activator receptor-related peptide-1 (SLURP-1), a positive allosteric modulator of alpha7 nAChR signaling. Our aim was to explore the roles played by SLURP-1 in the pathophysiology of asthma by assessing SLURP-1 expression in the OVA-sensitized murine asthma model and in cultured human bronchial epithelial cells. Using real-time PCR we found that expression of SLURP-1 mRNA is down-regulated in the lungs of asthmatic model mice, as compared to healthy mice. In addition, immunohistochemical studies confirmed the diminished expression of SLURP-1 in the bronchioles of asthmatic mice, and showed it was due to extensive metaplasia of mucus-secreting cells and the concomitant loss of ciliated epithelial cells. Expression of SLURP-1 mRNA and protein was also significantly down-regulated in human epithelial cells stimulat...Continue Reading

References

Apr 13, 2000·Pharmacology & Therapeutics·K Kawashima, T Fujii
Dec 19, 2001·The Journal of Clinical Investigation·J LaoukiliF Tournier
Oct 22, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yingjian YouSteven L Brody
Oct 25, 2002·American Journal of Respiratory Cell and Molecular Biology·Mitsuko KondoAtsushi Nagai
Mar 12, 2003·Life Sciences·Sergei A GrandoIgnaz Wessler
Sep 9, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Koichiro Kawashima, Takeshi Fujii
Apr 6, 2005·The Journal of Experimental Medicine·Rubina W SaeedChristine N Metz
Aug 6, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Robert VassalloLieping Chen
Aug 23, 2005·Proceedings of the American Thoracic Society·Peter J Barnes
Dec 16, 2005·The Journal of Investigative Dermatology·Juan ArredondoSergei A Grando
May 6, 2006·The Journal of Allergy and Clinical Immunology·Naomi YamashitaKen Ohta
Sep 30, 2006·The Journal of Investigative Dermatology·Bertrand FavreDaniel Hohl
Apr 7, 2007·American Journal of Respiratory Cell and Molecular Biology·Andrea J RossRobert B Devlin
May 1, 2007·Life Sciences·Sergei A GrandoIgnaz Wessler
Jun 29, 2007·Pharmacology & Therapeutics·Catherine R GwiltDuncan F Rogers
Aug 28, 2007·Current Opinion in Immunology·Taylor Doherty, David Broide
Feb 29, 2008·Annual Review of Immunology·Benjamin D MedoffAndrew D Luster
May 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Neerad C MishraMohan L Sopori
Jun 21, 2008·Histochemistry and Cell Biology·W KummerU Pfeil
Aug 2, 2008·The European Respiratory Journal·H MeursJ Zaagsma
Sep 1, 2009·Cellular Immunology·Yukiko MatsuoNaomi Yamashita

❮ Previous
Next ❯

Citations

Mar 19, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Andres MatosoMurray B Resnick
Apr 22, 2016·Human Genomics·Chelsea L LoughnerShivalingappa K Swamynathan
Nov 2, 2016·Applied Immunohistochemistry & Molecular Morphology : AIMM·Marcos LepeAndres Matoso
May 19, 2021·Archives of Biochemistry and Biophysics·Niyati A BorkarChristina M Pabelick

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.