Down regulation of SPAG9 reduces growth and invasive potential of triple-negative breast cancer cells: possible implications in targeted therapy

Journal of Experimental & Clinical Cancer Research : CR
Abhilasha SinhaAnil Suri

Abstract

Recently, we reported an association of a novel cancer testis (CT) antigen, sperm-associated antigen 9 (SPAG9) expression in breast cancer clinical samples, indicating its potential role in carcinogenesis. Around 15% breast cancers are designated as triple-negative for which treatment modalities are limited. Therefore, in the present study, we assessed the role of SPAG9 in triple-negative breast cancer cells. SPAG9 mRNA and protein expression was investigated in various breast cancer cells of different hormone receptor status and different subtypes by employing reverse transcriptase-polymerase chain reaction (RT-PCR), real time PCR, Western blotting, indirect immunofluorescence (IIF) and fluorescence activated cell sorting (FACS). Employing plasmid-based small interfering RNA (siRNA) approach, knockdown of SPAG9 was carried out in triple-negative breast cancer cells, MDA-MB-231, to assess its role on various malignant properties in vitro and in vivo. SPAG9 mRNA and protein expression was detected in all breast cancer cells. Further, IIF results showed that SPAG9 was predominantly localized in the cytoplasm of breast cancer cells. FACS analysis revealed distinct SPAG9 surface localization in breast cancer cells. Gene silencing o...Continue Reading

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Citations

Dec 4, 2015·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Chuang YangQifan Zhang
Jul 28, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Nirmala JagadishAnil Suri
Jun 9, 2019·Journal of Experimental & Clinical Cancer Research : CR·Honghong ShenChen Wang
Jan 23, 2016·Journal of Experimental & Clinical Cancer Research : CR·Guohua LouYanning Liu
Feb 20, 2016·Experimental and Therapeutic Medicine·Xiaorong YangShiqing Liu
Jul 2, 2019·Molecular Pharmaceutics·Liziane O F MonteiroElaine A Leite
Jun 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun PanLijun Mao

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Methods Mentioned

BETA
xenograft
PCR
electrophoresis
fluorescence activated cell sorting
FACS
transfection

Software Mentioned

SPSS
CellQuest

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