Down-regulation of specific plastid ribosomal proteins suppresses thf1 leaf variegation, implying a role of THF1 in plastid gene expression

Photosynthesis Research
Zhaoxue MaJirong Huang

Abstract

Chloroplast development is regulated by many biological processes. However, these processes are not fully understood. Leaf variegation mutants have been used as powerful models to elucidate the genetic network of chloroplast development since the degree of leaf variegation is regulated by developmental and environmental cues. The thylakoid formation 1 (thf1) mutant is unique for its variegation in both leaves and cotyledons. Here, we reported a new suppressor gene of thf1 leaf variegation, designated sot8. Map-based cloning and DNA sequencing results showed that a single nucleotide mutation from G to A was detected in the second exon of the gene encoding the ribosomal protein small subunit 9 (PRPS9) in sot8-1, resulting in an amino acid change and a partial loss of PRPS9 function. However, sot8-1 was unable to rescue the thf1 phenotype in low photosystem II activity (Fv/Fm). In addition, we identified two T-DNA insertion mutants defective in plastid-specific ribosomal proteins (PSRPs), psrp2-1, and psrp5-1. Genetic analysis showed that knockdown of PSRP5 expression but not PSRP2 expression suppressed leaf variegation. Northern blotting results showed that precursors of plastid rRNAs over-accumulated in prps9-1 and psrp5-1, indi...Continue Reading

References

Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Jun 10, 2000·The Plant Journal : for Cell and Molecular Biology·M ChenS Rodermel
Sep 5, 2001·The Plant Journal : for Cell and Molecular Biology·P PesaresiD Leister
Feb 28, 2003·European Journal of Biochemistry·Kenichi Yamaguchi, Alap R Subramanian
Mar 26, 2003·Genes & Genetic Systems·Wataru Sakamoto
Dec 17, 2003·The Plant Journal : for Cell and Molecular Biology·Zhengrong Ma, Hugo K Dooner
Mar 5, 2004·The Plant Journal : for Cell and Molecular Biology·Fei YuSteven R Rodermel
Feb 2, 2006·Journal of Experimental Botany·Maneesha R AluruSteven Rodermel
Sep 2, 2006·Nucleic Acids Research·Marcelo RogalskiRalph Bock
Feb 1, 2007·Plant, Cell & Environment·Fei YuSteven Rodermel
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Manjuli R SharmaRajendra K Agrawal
Jul 30, 2010·Journal of Integrative Plant Biology·Xiayan LiuSteve Rodermel
Aug 21, 2012·The Plant Journal : for Cell and Molecular Biology·Isidora RomaniPaolo Pesaresi
May 25, 2013·Photosynthesis Research·Aarthi PutarjunanSteve Rodermel
Oct 16, 2013·The Plant Journal : for Cell and Molecular Biology·Wenjuan WuJirong Huang

❮ Previous
Next ❯

Citations

Aug 25, 2016·Molecular Microbiology·Jiao ZhanQiang Wang
Sep 3, 2016·Molecular Plant·Kenji NishimuraWataru Sakamoto
Aug 6, 2019·Advanced Science·Yi Zhang, Congming Lu
Apr 20, 2020·Molecular Genetics and Genomics : MGG·Xiaobin ZhaoJinxing Tu
Mar 7, 2021·International Journal of Molecular Sciences·Dandan ZhuHeqin Zhu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.