Abstract
Mammalian Sprouty2 (Spry2) is a key regulator of the receptor tyrosine kinase/ERK signaling pathway and involved in many biological processes, including cell growth, migration, and tumor suppression. Here, we demonstrated that the intracellular protein level of Spry2 was significantly down-regulated by tumor necrosis factor-alpha (TNF-alpha) in both murine Swiss 3T3 fibroblasts and MLE15 lung epithelial cells. Although TNF-alpha activates multiple signaling cascades, only the inhibitor of p38 MAPK pathway blocked TNF-alpha-induced Spry2 down-regulation. Moreover, since both the mRNA level and protein half-life of Spry2 were unaltered by TNF-alpha treatment, this indicated the possible involvement of a translational mechanism in mediating the inhibitory effect of TNF-alpha. Importantly, rescue of the TNF-alpha-induced down-regulation of Spry2 by gene overexpression led to reverse of the apoptotic effect of TNF-alpha in Swiss 3T3 cells. To our knowledge, this study is the first that reported the association of Spry2 with TNF-alpha signaling pathway.
References
Jan 1, 1992·Annual Review of Immunology·P Vassalli
Aug 22, 1997·The Journal of Biological Chemistry·J W PierceM E Gerritsen
Feb 11, 1998·Cell·N HacohenM A Krasnow
Mar 25, 1999·Cell·T CasciM Freeman
Mar 30, 2001·The Journal of Biological Chemistry·Y YigzawT B Patel
Aug 22, 2001·Trends in Cell Biology·V Baud, M Karin
Oct 5, 2001·The Journal of Biological Chemistry·I GrossJ D Licht
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·James E EganDafna Bar-Sagi
Nov 5, 2002·The Journal of Biological Chemistry·Yinges YigzawTarun B Patel
Mar 26, 2003·Cell Death and Differentiation·H WajantP Scheurich
Jun 28, 2003·Journal of Cell Science·Graeme R GuyChee Wai Fong
Jan 18, 2005·American Journal of Respiratory Cell and Molecular Biology·Deborah E SullivanArnold R Brody
Feb 11, 2005·American Journal of Physiology. Endocrinology and Metabolism·David L WilliamsonLeonard S Jefferson
Dec 13, 2005·Trends in Cell Biology·Jacqueline M MasonJonathan D Licht
Dec 24, 2005·The Journal of Biological Chemistry·Francis EdwinTarun B Patel
Feb 14, 2006·Cancer Letters·Ting Ling LoGraeme R Guy
Dec 13, 2006·Metabolism: Clinical and Experimental·Charles H Lang, Robert A Frost
Mar 23, 2007·The Biochemical Journal·Christopher G Proud
May 23, 2007·Journal of Cellular Physiology·Wei DingDavid Warburton
Dec 12, 2007·The Journal of Biological Chemistry·Francis Edwin, Tarun B Patel
Citations
Dec 13, 2012·Angiogenesis·Martin PeierRok Humar
Jul 3, 2009·Molecular Pharmacology·Francis EdwinTarun B Patel
Sep 24, 2010·PLoS Genetics·Xiao Ou ShuFrank B Hu
Apr 20, 2014·Cancer Metastasis Reviews·Samar Masoumi-MoghaddamDavid Lawson Morris
Feb 19, 2013·International Journal of Food Microbiology·Kevin J AllenLili R Mesak
Nov 4, 2016·Neurochemical Research·Ning-Ning ChenShaoyu Liu
Mar 7, 2019·Bioscience Reports·Feifei CheJiao Chen
Feb 7, 2021·Nature Communications·Michael A SchumacherMark R Frey