Down-regulation of the c-Jun N-terminal kinase (JNK) phosphatase M3/6 and activation of JNK by hydrogen peroxide and pyrrolidine dithiocarbamate

Oncogene
Y R ChenT H Tan

Abstract

Oxidative stress activates the c-Jun N-terminal kinase (JNK) pathway. However, the exact mechanisms by which reactive oxygen species (ROS) activate JNK are unclear. We found that the ability of hydrogen peroxide (H(2)O(2)) to induce JNK activation varied in different cell types. Pyrrolidine dithiocarbamate (PDTC), a presumed antioxidant, induced JNK activation on its own and enhanced JNK activation by H(2)O(2) in many cell types, including Jurkat, HEK293, and LNCaP and Tsu-Pr1 prostate cancer cells. The activation of JNK by PDTC, in the presence or absence of exogenous H(2)O(2), was dependent on its chelating ability to metal ions, most likely copper ions. Despite the strong JNK-activating ability, H(2)O(2) plus PDTC did not induce significant activation of the upstream kinases, SEK1/MKK4 and MKK7. However, the JNK inactivation rate was slower in cells treated with H(2)O(2) plus PDTC compared with the rate in cells treated with ultraviolet C (UV-C). Treatment of H(2)O(2) plus PDTC significantly decreased the expression levels of a JNK phosphatase, M3/6 (also named hVH-5), but not the levels of other phosphatases (PP2A and PP4). In contrast, UV-C irradiation did not cause the down-regulation of M3/6. These results suggest that J...Continue Reading

References

May 1, 1992·The Journal of Experimental Medicine·R SchreckP A Baeuerle
Jan 1, 1990·Methods in Enzymology·B Halliwell, J M Gutteridge
Apr 1, 1984·The Biochemical Journal·B Halliwell, J M Gutteridge
Dec 1, 1994·Molecular and Cellular Biology·H K SlussR J Davis
May 12, 1994·Nature·J M KyriakisJ R Woodgett
Jan 12, 1996·The Journal of Biological Chemistry·Y R ChenT H Tan
Oct 18, 1996·The Journal of Biological Chemistry·P Gómez del ArcoJ M Redondo
Jan 1, 1997·Journal of Clinical Pathology·J A SnowdenK P West
Mar 7, 1998·The Journal of Biological Chemistry·Y LuoG S Roth
Apr 30, 1998·Current Opinion in Cell Biology·Y T Ip, R J Davis
Jul 2, 1998·Biochemical and Biophysical Research Communications·J ChenS L Schreiber
Mar 4, 1999·The EMBO Journal·V AdlerZ Ronai
Mar 19, 1999·Molecular and Cellular Biology·H J Schaeffer, M J Weber

❮ Previous
Next ❯

Citations

Apr 20, 2004·Assay and Drug Development Technologies·Christopher J HoweRichard A Franklin
May 7, 2002·EMBO Reports·Moran BenharAlexander Levitzki
Mar 30, 2006·Science's STKE : Signal Transduction Knowledge Environment·Michael Y Sherman, Vladimir L Gabai
Feb 16, 2008·American Journal of Respiratory Cell and Molecular Biology·Alessandra Rinna, Henry Jay Forman
Jul 10, 2012·Cell & Bioscience·Ching-Yu Huang, Tse-Hua Tan
Nov 12, 2013·Antioxidants & Redox Signaling·Pablo RíosRafael Pulido
Apr 24, 2007·Free Radical Biology & Medicine·Zachary A PfeifferPaul J Bertics
Jul 22, 2008·Cancer Letters·Wei-Qun DingStuart E Lind
Jun 5, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Liang Zhang, Li Cui
Jul 22, 2010·Journal of Cellular Physiology·Jaya DasguptaJ Andres Melendez
May 8, 2004·Biochemical Pharmacology·Byeong-Churl JangSeong-Il Suh
Mar 13, 2014·Journal of the American College of Cardiology·Dimitris TousoulisChristodoulos Stefanadis
Mar 24, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hang QiYoshimasa Nakamura
Dec 6, 2014·BMC Complementary and Alternative Medicine·Norsharina IsmailMuhammad Firdaus Abu Bakar
Jan 9, 2007·Biochimica Et Biophysica Acta·Kunio Kondoh, Eisuke Nishida
Oct 25, 2016·Biochemical and Biophysical Research Communications·Hema NegiRakesh Pandey
Jul 13, 2002·Journal of Cellular Physiology·Jennifer L Martindale, Nikki J Holbrook
May 27, 2016·Circulation Research·Ruijie LiuJeffery D Molkentin
Apr 8, 2006·Physiological Reviews·Alain Tedgui, Ziad Mallat
Apr 15, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Mark LehnertJohn J Lemasters
Jun 4, 2019·International Journal of Molecular Sciences·Hsueh-Fen ChenTse-Hua Tan
Mar 12, 2017·Molecular Pharmacology·Yoshiki KobayashiPeter J Barnes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.