PMID: 9523572Apr 2, 1998Paper

Down-regulation of the human norepinephrine transporter in intact 293-hNET cells exposed to desipramine

Journal of Neurochemistry
M Y ZhuG A Ordway

Abstract

The effects of continuous exposure of cultured cells expressing the human norepinephrine transporter (hNET) to the hNET inhibitor desipramine on hNET expression and function were studied. Exposure of HEK-293 cells transfected stably with the hNET cDNA (293-hNET cells) to desipramine for 3 days reduced the specific binding of [3H]nisoxetine in membrane homogenates in a concentration-dependent manner. The magnitude of the reductions in [3H]nisoxetine binding to hNET was dependent on the length of time of the exposure to desipramine, reaching 77% after a 21-day exposure. The reduction of [3H]nisoxetine binding returned to control levels within 72 h after a 3-day exposure to desipramine. Reductions in [3H]nisoxetine binding to hNET were accompanied by time-dependent and exposure concentration-dependent reductions in hNET protein levels as determined by western blotting. Similar to binding, hNET protein levels returned to control levels 72 h after cessation of desipramine exposure. Northern blotting indicated that exposure of 293-hNET cells to desipramine did not significantly alter hNET mRNA levels. Uptake of [3H]norepinephrine by 293-hNET cells was markedly reduced after a 3-day exposure to desipramine. However, desipramine exposu...Continue Reading

Citations

Jan 5, 1999·The Journal of Biological Chemistry·E M Bernstein, M W Quick
May 19, 2000·The Journal of Biological Chemistry·R M LawM W Quick
Mar 17, 2006·Journal of Neurochemistry·Prashant Mandela, Gregory A Ordway
Aug 24, 2007·The European Journal of Neuroscience·Alexis M Jeannotte, Anita Sidhu
Jan 25, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Thorsten LauPatrick Schloss
Mar 12, 2008·Journal of Neurochemistry·Alexis M Jeannotte, Anita Sidhu
Apr 18, 2008·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Zaorui ZhaoJames M O'Donnell
Oct 17, 2008·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Zaorui ZhaoJames M O'Donnell
Jan 4, 2003·Nature Reviews. Neuroscience·Gonzalo E TorresMarc G Caron
Feb 14, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Weike MaoChang-seng Liang
Jun 14, 2000·Current Opinion in Neurobiology·R D Blakely, A L Bauman
Jun 15, 2021·European Journal of Pharmacology·Renata WolińskaMagdalena Bujalska-Zadrożny

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