Downexpression of HSD17B6 correlates with clinical prognosis and tumor immune infiltrates in hepatocellular carcinoma

Cancer Cell International
Lei LvQiyi Yi

Abstract

Hydroxysteroid 17-Beta Dehydrogenase 6 (HSD17B6), a key protein involved in synthetizing dihydrotestosterone, is abundant in the liver. Previous studies have suggested a role for dihydrotestosterone in modulating progress of various malignancies, and HSD17B6 dysfunction was associated with lung cancer and prostate cancer. However, little is known about the detailed role of HSD17B6 in hepatocellular carcinoma (HCC). Clinical implication and survival data related to HSD17B6 expression in patients with HCC were obtained through TCGA, ICGC, ONCOMINE, GEO and HPA databases. Survival analysis plots were drawn with Kaplan-Meier Plotter. The ChIP-seq data were obtained from Cistrome DB. Protein-Protein Interaction and gene functional enrichment analyses were performed in STRING database. The correlations between HSD17B6 and tumor immune infiltrates was investigated via TIMER and xCell. The proliferation, migration and invasion of liver cancer cells transfected with HSD17B6 were evaluated by the CCK8 assay, wound healing test and transwell assay respectively. Expression of HSD17B6, TGFB1 and PD-L1 were assessed by quantitative RT-PCR. HSD17B6 expression was lower in HCC compared to normal liver and correlated with tumor stage and grade....Continue Reading

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Datasets Mentioned

BETA
GSE25097
GSE14520
GSE5230
GSE35313

Methods Mentioned

BETA
RNA-Seq
transfecting
PCR
protein assay
ChIP-seq
immunoprecipitation
transfection

Software Mentioned

xCell
TIMER “ DiffExp
PRISM
GraphPad
Xena Functional Genomics Explorer
Gene Set Enrichment Analysis ( GSEA )
Search Tool for the Retrieval of Genes ( STRING
GTEx
GSEA
HCCDB

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