Downhill protein folding modules as scaffolds for broad-range ultrafast biosensors

Journal of the American Chemical Society
Michele CerminaraVictor Muñoz

Abstract

Conformational switches are macromolecules that toggle between two states (active/inactive or folded/unfolded) upon specific binding to a target molecule. These molecular devices provide an excellent scaffold for developing real-time biosensors. Here we take this concept one step beyond to build high-performance conformational rheostat sensors. The rationale is to develop sensors with expanded dynamic range and faster response time by coupling a given signal to the continuous (rather than binary) unfolding process of one-state downhill folding protein modules. As proof of concept we investigate the pH and ionic-strength sensing capabilities of the small α-helical protein BBL. Our results reveal that such a pH/ionic-strength sensor exhibits a linear response over 4 orders of magnitude in analyte concentration, compared to the 2 orders of magnitude for switches, and nearly concentration-independent microsecond response times.

References

May 20, 1998·Trends in Biotechnology·H W Hellinga, J S Marvin
Mar 1, 1996·Nature Biotechnology·S Tyagi, F R Kramer
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·B A ShoemakerP G Wolynes
Aug 29, 2001·Nature Structural Biology·J S Marvin, H W Hellinga
Dec 14, 2002·Science·Maria M Garcia-MiraVictor Muñoz
May 9, 2003·Nature·Wei Yuan Yang, Martin Gruebele
Aug 9, 2003·Analytical and Bioanalytical Chemistry·Matthew A Cooper
Jul 15, 2004·Journal of the American Chemical Society·Fabiana Y Oliva, Victor Muñoz
Dec 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Victor Muñoz, Jose M Sanchez-Ruiz
Jul 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jonathan E Kohn, Kevin W Plaxco
Jun 27, 2006·Nature·Mourad SadqiVictor Muñoz
Jul 13, 2006·Biochemistry·Athi N NaganathanVictor Muñoz
Feb 13, 2007·Annual Review of Biophysics and Biomolecular Structure·Victor Muñoz
Apr 11, 2007·Journal of the American Chemical Society·Athi N NaganathanVictor Muñoz
Oct 25, 2008·ACS Chemical Biology·Margaret M StrattonStewart N Loh
Jan 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Peng LiVictor Muñoz
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Alexis Vallée-BélisleKevin W Plaxco
Aug 24, 2010·The Journal of Biological Chemistry·Tanay M DesaiVictor Muñoz
Nov 26, 2010·Trends in Biotechnology·Kevin W Plaxco, H Tom Soh
Oct 29, 2011·Science·Kresten Lindorff-LarsenDavid E Shaw
Dec 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jianwei LiuVictor Muñoz
Jan 14, 2012·Journal of the American Chemical Society·Alexis Vallée-BélisleKevin W Plaxco

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Citations

Mar 20, 2014·Quarterly Reviews of Biophysics·Hannah Gelman, Martin Gruebele
Feb 5, 2016·Current Opinion in Structural Biology·Victor MuñozMourad Sadqi
Mar 28, 2017·The Journal of Physical Chemistry Letters·Abhishek Narayan, Athi N Naganathan
May 30, 2017·ACS Applied Materials & Interfaces·Lydia KisleyDeborah E Leckband
Oct 19, 2017·Physical Chemistry Chemical Physics : PCCP·Xiakun Chu, Victor Muñoz
Aug 31, 2016·The Biochemical Journal·Victor Muñoz, Michele Cerminara
Dec 20, 2016·Journal of the American Chemical Society·Abhishek NarayanAthi N Naganathan
Sep 12, 2020·Accounts of Chemical Research·Luis Alberto CamposVictor Muñoz

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