Downregulated GBX2 gene suppresses proliferation, invasion and angiogenesis of breast cancer cells through inhibiting the Wnt/β-catenin signaling pathway.

Cancer Biomarkers : Section a of Disease Markers
Ying FangSai-Nan Hu

Abstract

Gastrulation brain homeobox 2 (GBX2), a gene involved in mid/hindbrain region, has been revealed as one of the oncogene associated with certain cancers, as an example being prostate cancer. However, despite years of worldwide research, the underlying mechanism of GBX2 as well as its significance in breast cancer still remains unclear. Therefore, the present study evaluates the abilities of GBX gene silencing providing for the proliferation, invasion and angiogenesis of breast cancer cells by way of the Wnt/β-catenin signaling pathway. We employed a microarray analysis to screen out differentially expressed genes relative to breast cancer. Moreover, we retrieved GBX2 expression in breast cancer to find out the relationship between GBX2 expression and prognosis in breast cancer. We performed RT-qPCR to screen out cell lines with high GBX2 expression. Subsequently, both RT-qPCR and western blot analysis were employed so as to measure the combination of the mRNA and protein expressions of GBX2, β-catenin, vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-2, and MMP-9. The effect that GBX2 gene silencing and the Wnt/β-catenin signaling pathway had on cell proliferation, invasion, angiogenesis, and tumorigenic...Continue Reading

References

Jul 25, 2008·Clinical Breast Cancer·Stephen E Jones
Jan 16, 2010·Biochemical and Biophysical Research Communications·Ying TengDaoxin Ma
Jan 30, 2010·Cancer Metastasis Reviews·Kelly K Haagenson, Gen Sheng Wu
Apr 17, 2010·The American Journal of Pathology·Andrey I KhramtsovKathleen H Goss
Jan 13, 2011·Cancer Research·Dong Woo KangDo Sik Min
Aug 10, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Julienne E BowerSteve W Cole
Nov 9, 2011·Nature Medicine·Sara M Weis, David A Cheresh
May 29, 2012·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Nan SongDaehee Kang
Mar 29, 2013·Nature Genetics·Kyriaki MichailidouDouglas F Easton
Apr 4, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ke-Da YuJenny C Chang
Aug 3, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Wentao LiBaoping Zhai
Mar 12, 2016·OncoTargets and Therapy·Sławomir ŁawickiMaciej Szmitkowski
Oct 25, 2016·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Sławomir ŁawickiMaciej Szmitkowski
Apr 22, 2017·The International Journal of Eating Disorders·Jake Linardon, Leah Brennan
Nov 24, 2017·Cancer Treatment Reviews·Nithya Krishnamurthy, Razelle Kurzrock
Nov 25, 2017·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Qingnan ZhaoYanSong Li
Jan 1, 2018·Differentiation; Research in Biological Diversity·Zhe WangKyo Yamasu
Feb 2, 2018·Progress in Molecular Biology and Translational Science·Caroline M Alexander
Feb 3, 2018·OncoTargets and Therapy·Zahid KhanMohammad Saud Alanazi

❮ Previous
Next ❯

Citations

Feb 15, 2019·Journal of Cellular and Molecular Medicine·Zheng WangLiang Han
Feb 8, 2021·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Xudong ChenCihao Hu

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE33447
GPL14550

Methods Mentioned

BETA
chip
surgical resection
transfection
PCR
electrophoresis
enzyme-linked immunosorbent assay
xenograft

Software Mentioned

R Programming Language
limma
Image J
SPSS
UALCAN
pheatmap

Related Concepts

Related Feeds

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.