Downregulated lincRNA HOTAIR expression in ovarian cancer stem cells decreases its tumorgeniesis and metastasis by inhibiting epithelial-mesenchymal transition

Cancer Cell International
Jing WangJ Dou

Abstract

Emerging evidence indicates that dysregulated long intervening non-coding RNA (lincRNA) HOTAIR correlates highly with tumor invasion and metastasis but a link between the high expression of HOTAIR and the metastatic cascade of cancer stem cells (CSCs) needs to be further studied. The purpose of this study was to investigate the effect of down-regulated HOTAIR expression on tumorgeniesis and metastasis of epithelial ovarian cancer (EOC) CSCs. CD117(+)CD44(+)CSCs were isolated from human EOC SKOV3 cell line by using a magnetic-activated cell sorting system, and were then transfected with the expression vector-based small hairpin RNA targeting HOTAIR; the stably transfected cells were selected for the study. Colony-forming, wound-healing, cellular metastasis and tumorigenicity assays were performed. The results demonstrated that the HOTAIR expression in clinical EOC tissues and SKOV3 CD117(+)CD44(+)CSCs was higher than in SKOV3 tumor tissues and non-CD117(+)CD44(+)CSCs. The CD117(+)CD44(+)-shHOTAIR showed an inhibited HOTAIR expression, reduced cell migration and invasion than CD117(+)CD44(+)- scramble, suggesting the inhibition of an epithelial-mesenchymal transition. Moreover, the downregulated HOTAIR expression in CD117(+)CD44(...Continue Reading

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Citations

Dec 1, 2017·Journal of Experimental & Clinical Cancer Research : CR·Qiaohua PengSongfa Zhang
Nov 13, 2018·Stem Cells and Development·Deanne J WhitworthErnst J Wolvetang
May 1, 2019·Journal of Cellular Biochemistry·Nashwa El-KhazragyOmar Khalifa
Sep 2, 2017·Scientific Reports·Bo HanNing Yang
Jul 18, 2018·Pathology Oncology Research : POR·Xi-Ping ZhangEn-Qi Qiao
Jun 27, 2017·Cancer Cell International·Jing LiZhongfu Yuan
Dec 22, 2020·Seminars in Cancer Biology·Evan M McCabe, Theodore P Rasmussen
Apr 1, 2021·Molecular Cancer Therapeutics·Weini WangKenneth P Nephew
May 30, 2021·Translational Oncology·Melanie SchwerdtfegerMichele Caraglia

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Methods Mentioned

BETA
reverse transcription-PCR
PCR
electrophoresis
xenograft

Software Mentioned

BLAST
siDESIGN

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