Downregulated lncRNA UCA1 acts as ceRNA to adsorb microRNA-498 to repress proliferation, invasion and epithelial mesenchymal transition of esophageal cancer cells by decreasing ZEB2 expression

Cell Cycle
Peng WangJunxing Huang

Abstract

Objective: Esophageal cancer (EC) is one of the most general malignant tumors in humans. There were few studies researching the connections between lncRNA UCA1 and EC. This study is to research the effect of lncRNA UCA1 adsorbing microRNA-498 (miR-498) as a ceRNA to regulate ZEB2 expression on epithelial mesenchymal transition (EMT), invasion and migration of EC cells. Methods: UCA1, miR-498 and ZEB2 expression in EC tissues and cells was detected by RT-qPCR or western blot analysis. EC cells were transfected with siRNA-UCA1, miR-498 mimics or their controls to determine cell colony, proliferation, cycle distribution, apoptosis, migration and invasion by colony formation assay, CCK-8 assay, flow cytometry, and Transwell assay, respectively. The protein expression of PCNA, c-Myc, E-cadherin, N-cadherin, MMP-2 and MMP-9 was detected by Western blot analysis. The growth rate and weight of transplanted tumor in nude mice were observed. Results: There were overly expressed UCA1 and ZEB2 and lowly expressed miR-498 in EC tissues and cells. LncRNA UCA1 acted as ceRNA to inhibit miR-498 expression and thereby increasing ZEB2 expression. With down-regulated UCA1 and up-regulated miR-498, ZEB2 expression, cell proliferation, colony forma...Continue Reading

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Citations

Feb 29, 2020·International Journal of Molecular Sciences·Satoshi KubotaTakashi Nishida
Jul 17, 2020·Cancer Biotherapy & Radiopharmaceuticals·Ling Chen, Tiejun Zhao
Jan 20, 2021·Cellular and Molecular Life Sciences : CMLS·Siyuan LuanYong Yuan
Apr 23, 2021·Cancer Biotherapy & Radiopharmaceuticals·Weihe ZhouYi Jiang

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Datasets Mentioned

BETA
KYSE150

Methods Mentioned

BETA
transfection
PCR
electrophoresis
pull-down

Software Mentioned

Targetscan
ImageJ2x

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