Downregulated microRNA-340-5p promotes proliferation and inhibits apoptosis of chondrocytes in osteoarthritis mice through inhibiting the extracellular signal-regulated kinase signaling pathway by negatively targeting the FMOD gene

Journal of Cellular Physiology
Weikai ZhangHui Huang

Abstract

Osteoarthritis (OA) is a degenerative joint disease that leads to the destruction of joint function. The aim of this study is to investigate the effects of microRNA-340-5p (miR-340-5p) and its target gene, FMOD, on the proliferation and apoptosis of chondrocytes in mice with OA through the extracellular signal-regulated kinase (ERK) signaling pathway. Twenty healthy C57BL/6J mice aged 15 months with a weight of 50 ± 2 g were selected. Ten mice were treated using a unilateral knee anterior cruciate ligament transection as well as a medial meniscectomy to establish the OA model. Besides, another 10 mice were used as the control group. A reverse transcription quantitative polymerase chain reaction and Western blot analysis methods were used to examine the expressions of related genes in cells of each group. A 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide assay and flow cytometry were also conducted to evaluate the cell function after transfection had been completed. The expressions of fibromodulin (FMOD), type II collagen (Col II), B-cell lymphoma-2 (Bcl-2), sex-determining region of Y chromosome (SRY)-related high-mobility group-box gene 9 (Sox9), and proliferating cell nuclear antigen (PCNA) were decreased, wherea...Continue Reading

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Citations

Nov 30, 2019·Current Pharmaceutical Design·Su-Kang ShanLing-Qing Yuan
May 13, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Xijuan LiuJingyu Jia
Sep 14, 2019·International Journal of Molecular Sciences·Ehsan RazmaraSadegh Babashah
Jan 12, 2020·Wiley Interdisciplinary Reviews. RNA·Siyu JiangMin Yang
Jan 11, 2020·Frontiers in Pharmacology·Chenshuang LiMin Zou

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