Downregulation of β-actin gene and human antigen R in human keratoconus.

Investigative Ophthalmology & Visual Science
Roy JosephRoswell R Pfister

Abstract

The purpose of this study was to determine the expression levels and regulation of β-actin in the stroma of keratoconus (KC) and normal corneas. A total of 15 different human corneas from both KC and normal individuals were used for this study. Additionally, 3 Fuch's dystrophic corneas were also used. The β-actin gene expression was analyzed at the transcriptional and translational levels in the epithelium and stroma of the KC and normal corneas. The human antigen R (HuR) gene expression was analyzed by real-time PCR in the stroma of five KC and five normal corneas. The keratocytes from three normal and three KC corneas were cultured in the presence of serum, and the expression levels of β-actin and human antigen R (HuR) were analyzed by using confocal imaging in both normal and KC fibroblasts. The expression of the β-actin gene was downregulated in the stroma of the six KC corneas but not in the stroma of six normal and Fuchs' dystrophic corneas. Immunofluorescence detection of β-actin showed that it was absent in the KC fibroblast. The real-time PCR analysis of the HuR gene showed a relative 4.7-fold lower expression in KC corneas relative to the normal corneas, which was further confirmed by the immunofluorescence detection ...Continue Reading

Citations

May 2, 2015·Eye·V GalvisA Acera
May 3, 2016·Wiley Interdisciplinary Reviews. RNA·Soma DashSalil A Lachke
Aug 7, 2016·Eye and Vision·Krishnatej NishtalaArkasubhra Ghosh
Mar 3, 2017·BioMed Research International·Mariam Lotfy KhaledYutao Liu
Aug 31, 2019·Scientific Reports·Rabab SharifDimitrios Karamichos
Dec 14, 2018·Frontiers in Neuroscience·Marita Pietrucha-DutczakAdrian Smedowski
May 1, 2016·International Journal of Keratoconus and Ectatic Corneal Diseases·Yelena BykhovskayaYaron S Rabinowitz

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