Downregulation of ABCE1 via siRNA affects the sensitivity of A549 cells against chemotherapeutic agents

Medical Oncology
Göknur KaraEmir Baki Denkbaş

Abstract

ATP-binding cassette E1 (ABCE1) is involved in several biological functions in cancer cells such as tumor proliferation, antiapoptotic pathway and chemoresistance mechanism. This work aimed to investigate the alterations in chemosensitivity of A549 lung cancer cells for 5-Fluorouracil (5-FU) and irinotecan by silencing ABCE1 using specific small interfering RNAs (siRNA). The cells were treated with low doses of drugs, alone and also their combinations with ABCE1 siRNA. Cytotoxicity, cell proliferation and apoptosis/necrosis evaluations were performed in order to examine the effects of the combined treatment. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to confirm the downregulation of ABCE1. We also investigated the levels of B cell lymphoma 2 (Bcl-2) and mammalian target of rapamycin (mTOR) after the treatments by RT-PCR. Downregulation of ABCE1 improved the anticancer effects of 5-FU in inducing cell viability/proliferation inhibition and apoptosis/necrosis, whereas interestingly, almost did not change or slightly reduced the anticancer effects of irinotecan. ABCE1 expression significantly decreased by transfecting the cells with ABCE1 siRNA. Moreover, Bcl-2 and mTOR levels changed after the single or com...Continue Reading

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Jun 24, 2015·Critical Reviews in Oncology/hematology·M P WeenC Ricciardelli
Jul 16, 2019·Cancer Biomarkers : Section a of Disease Markers·Lihong WangPeng Xu
Jan 9, 2021·BMC Bioinformatics·Joshua D MannheimerDaniel L Gustafson

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