Downregulation of B7-H4 in the MHCC97-H hepatocellular carcinoma cell line by arsenic trioxide

Molecular Medicine Reports
Liming CuiWeizhe Zhang

Abstract

Arsenic trioxide (As2O3; ATO), a compound which is characterized by its ability to function as a potent anticancer agent, has been investigated in a variety of carcinomas. B7‑H4, a transmembrane protein, may inhibit the function of the T cell effector, and therefore, may be useful in investigating different types of tumor therapies. However, few studies have been published previously associated with the roles of ATO and B7‑H4 in human hepatocellular carcinoma (HCC). The aim of the present study was to investigate the anti‑invasive role of ATO in HCC, to determine the effect of ATO treatment on the expression of B7‑H4 and to further assess the possible underlying mechanisms. Following treatment of the cells with 2, 4 and 8 µM ATO for 48 h, cell counting kit‑8 (CCK‑8), Transwell and western blot assays were used to determine the extent of human MHCC97‑H HCC cell proliferation, apoptosis, invasion and B7‑H4 expression, respectively. The results revealed that 1 µM ATO markedly decreased cellular proliferation, and ATO administered at concentrations of 0.1, 0.2 and 0.5 µM markedly inhibited the migration and invasion of the human MHCC97‑H HCC cell line. The expression of B7‑H4 in the treatment groups was markedly reduced. Signal tra...Continue Reading

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Nov 19, 2019·Current Cancer Drug Targets·Md WahiduzzamanYoshitaka Hosokawa
Dec 10, 2020·Expert Opinion on Drug Delivery·Vikas KumarHyung Sik Kim
Nov 18, 2019·Cellular Immunology·Jia-Yu Wang, Wei-Peng Wang

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Methods Mentioned

BETA
surgical resection
flow cytometry

Software Mentioned

BD [UNK]
SPSS

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