Downregulation of basic fibroblast growth factor is associated with femoral head necrosis in broilers

Poultry Science
P F LiJ F Hou

Abstract

Femoral head necrosis (FHN) is a metabolic cartilage disease of rapidly growing broilers. The aim of the present study was to investigate the role of basic fibroblast growth factor (bFGF) in the apoptotic processes associated with FHN. Broilers were selected and categorized based on clinical examination in 3 groups: healthy, femoral head separation, or femoral head separation with growth plate lacerations. Hematoxylin and eosin staining showed fewer chondrocytes in the resting zone of the growth plates when FHN occurred. Moreover, the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay revealed a significant increase in chondrocyte apoptosis. Furthermore, immunohistochemical assays and real-time quantitative PCR analysis demonstrated a decline in bFGF expression. In addition, reduced Bcl-2 mRNA expression was observed along with a corresponding increase in Bax and caspase-3 mRNA expression in FHN samples. There was a correlation between bFGF protein expression and the proportion of TUNEL-positive cells and a correlation between bFGF mRNA expression and expression of Bax, and caspase-3. The results of the study suggested that the expression of bFGF was reduced in the process of chondrocyte apoptosis, which...Continue Reading

References

Jul 10, 1999·The Journal of Biological Chemistry·B B Wolf, D R Green
Feb 25, 2000·The International Journal of Biochemistry & Cell Biology·M A Nugent, R V Iozzo
Feb 13, 2001·The Journal of Clinical Investigation·U I ChungH M Kronenberg
May 16, 2003·Nature·Henry M Kronenberg
Dec 1, 2004·The Journal of Bone and Joint Surgery. British Volume·J D F CalderJ M Polak
Apr 16, 2005·Critical Reviews in Eukaryotic Gene Expression·Olivia FromiguePierre J Marie
May 19, 2005·Journal of Comparative Pathology·N C RathJ M Balog
Apr 18, 2007·Molecular and Cellular Biology·Mi-Yoon ChangSang-Hun Lee
Jun 15, 2007·Journal of Cellular Physiology·Dimitrios AgasMaria Giovanna Sabbieti
Feb 7, 2008·PloS One·Toby G KnowlesChristine J Nicol
Dec 25, 2008·Journal of Cellular Physiology·Maria Giovanna SabbietiMarja M Hurley
Aug 1, 2000·Avian Pathology : Journal of the W.V.P.A·P T McNamee, J A Smyth
May 2, 2009·Applied Immunohistochemistry & Molecular Morphology : AIMM·Chao-Jie WangXiao-Feng Sun
Nov 9, 2010·Cell Biology International·Dapeng P JinYuehui Ma
Dec 23, 2011·Journal of Orthopaedic Surgery and Research·Mohammad Amin KerachianChantal Séguin
May 7, 2013·Journal of Zhejiang University. Science. B·Lei TianKun-zheng Wang

❮ Previous
Next ❯

Citations

Oct 20, 2015·Avian Diseases·B PackialakshmiG R Huff
Nov 25, 2016·Animal : an International Journal of Animal Bioscience·E PaludoM C Ledur
Jul 12, 2020·International Journal of Molecular Sciences·Yaling YuZhenlei Zhou
Jul 2, 2021·Frontiers in Physiology·Rubin FanZhenlei Zhou

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Yuriko HirotaJinichi Fukuda
Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Hae Jeong ParkJ-H Chung
© 2021 Meta ULC. All rights reserved