Downregulation of CDK-8 inhibits colon cancer hepatic metastasis by regulating Wnt/β-catenin pathway

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Wen-Song CaiJie Cao

Abstract

Liver metastasis is a major cause of mortality from colon cancer. To investigate the role of cyclin-dependent kinase 8 (CDK8) in the progression of colon cancer hepatic metastasis. In this present study, human colon cancer HCT116 or HCT116-LUC-GFP cells were transfected with Lentiviral vector-mediated knockdown of CDK-8. After transfection, metastasis and invasion potential of colon cancer cell was investigated by wound healing and transwell invasion assays, respectively. A mice model of colon cancer liver metastases was established and observed with bioluminescence imaging. The protein expression of CDK-8, β-catenin, E2F1, MMP-7 and E-cadherin in liver tissues were detected by Western Blot. Our results revealed that lentiviral vector-mediated knockdown of CDK-8 inhibited metastasis and invasion of colon cancer cells in vitro and in vivo, respectively. Protein expression of CDK-8, β-catenin, MMP-7 and E-cadherin were inhibited, but protein expression of E2F1 was enhanced. In sum, our data provided compelling evidence that CDK-8 played a significant role in colon cancer hepatic metastasis by regulating the Wnt/β-catenin signal pathway and might sever as a potential therapeutic target for colon cancer patients.

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Citations

Aug 24, 2017·The American Journal of Chinese Medicine·Yo-Han HanSeung-Heon Hong
Sep 30, 2020·Medicinal Research Reviews·Asiyeh JebelliLobat Tayebi
Feb 2, 2018·Cancer Immunology Research·Agnieszka Witalisz-SieprackaVeronika Sexl
Dec 2, 2020·European Journal of Medicinal Chemistry·Dan WuXinhua Liu

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