Downregulation of chloroplast RPS1 negatively modulates nuclear heat-responsive expression of HsfA2 and its target genes in Arabidopsis.

PLoS Genetics
Hai-Dong YuFang-Qing Guo

Abstract

Heat stress commonly leads to inhibition of photosynthesis in higher plants. The transcriptional induction of heat stress-responsive genes represents the first line of inducible defense against imbalances in cellular homeostasis. Although heat stress transcription factor HsfA2 and its downstream target genes are well studied, the regulatory mechanisms by which HsfA2 is activated in response to heat stress remain elusive. Here, we show that chloroplast ribosomal protein S1 (RPS1) is a heat-responsive protein and functions in protein biosynthesis in chloroplast. Knockdown of RPS1 expression in the rps1 mutant nearly eliminates the heat stress-activated expression of HsfA2 and its target genes, leading to a considerable loss of heat tolerance. We further confirm the relationship existed between the downregulation of RPS1 expression and the loss of heat tolerance by generating RNA interference-transgenic lines of RPS1. Consistent with the notion that the inhibited activation of HsfA2 in response to heat stress in the rps1 mutant causes heat-susceptibility, we further demonstrate that overexpression of HsfA2 with a viral promoter leads to constitutive expressions of its target genes in the rps1 mutant, which is sufficient to reestab...Continue Reading

References

Jan 1, 1988·Annual Review of Genetics·S Lindquist, E A Craig
May 24, 1983·Biochemistry·T Suryanarayana, A R Subramanian
Dec 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·J KropatC F Beck
Feb 3, 1999·Annual Review of Genetics·M SugiuraM Sugita
Feb 18, 1999·Molecular and Cellular Biology·S P GygiR Aebersold
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y KovtunJ Sheen
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·S W Hong, E Vierling
Aug 23, 2000·Nucleic Acids Research·A Shteiman-Kotler, G Schuster
Feb 16, 2002·The Plant Journal : for Cell and Molecular Biology·Teruaki TajiKazuo Shinozaki
May 9, 2002·Journal of Experimental Botany·Steven J NeillJohn T Hancock
May 9, 2002·Journal of Experimental Botany·Shigeru ShigeokaKazuya Yoshimura
Sep 5, 2002·The Plant Journal : for Cell and Molecular Biology·Veena SangwanRajinder S Dhindsa
Dec 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Haejeong MoonDae-Jin Yun
Jan 31, 2004·Plant Molecular Biology·Livia MerendinoJean-David Rochaix
Sep 21, 2004·Annual Review of Plant Biology·Klaus Apel, Heribert Hirt
Oct 7, 2004·Trends in Plant Science·Ron MittlerFrank Van Breusegem
Dec 22, 2004·The Plant Journal : for Cell and Molecular Biology·Wolfgang BuschFritz Schöffl
Dec 31, 2004·Journal of Biosciences·Sanjeev Kumar BaniwalPascal von Koskull-Döring

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Citations

Dec 18, 2013·Plant Physiology and Biochemistry : PPB·Masanori SatohShinji Masuda
Jul 16, 2013·Journal of Plant Physiology·Magdalena Szechyńska-Hebda, Stanisław Karpiński
Aug 28, 2012·Plant Science : an International Journal of Experimental Plant Biology·Ching-Hui YehYee-Yung Charng
Oct 27, 2015·Frontiers in Plant Science·Krzysztof Bobik, Tessa M Burch-Smith
Aug 16, 2014·Plant, Cell & Environment·Sotirios FragkostefanakisEnrico Schleiff
Apr 12, 2016·Frontiers in Plant Science·Ai-Zhen Sun, Fang-Qing Guo
Mar 11, 2015·The Plant Journal : for Cell and Molecular Biology·Michael SchrodaTimo Mühlhaus
Jan 15, 2015·Biochimica Et Biophysica Acta·Tatjana Kleine, Dario Leister
Mar 15, 2018·International Journal of Molecular Sciences·Qing-Long WangFang-Qing Guo
Mar 1, 2013·International Journal of Molecular Sciences·Valeria BantiPierdomenico Perata
Apr 17, 2019·Journal of Experimental Botany·Vivek DograChanhong Kim
Jun 16, 2017·Proceedings. Biological Sciences·David L Des MaraisSamuel V Scarpino
Oct 12, 2019·Scientific Reports·Siting ChenMingliu Yang
Apr 6, 2017·Frontiers in Plant Science·Dario LeisterTatjana Kleine
Jul 19, 2018·Frontiers in Plant Science·Yue Niu, Yun Xiang
Aug 28, 2020·International Journal of Molecular Sciences·Yi ZhangCongming Lu
May 1, 2019·Annual Review of Plant Biology·Jingyu ZhangKang Chong
Apr 18, 2020·Frontiers in Plant Science·Shanshan HuCheng Zhu
Oct 16, 2020·Plant, Cell & Environment·Fernanda MarchettiGabriela Carolina Pagnussat

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Datasets Mentioned

BETA
M82923
AJ585191
and

Methods Mentioned

BETA
transgenic
sumoylation
electrophoresis
environmental stress
transmission electron microscopy
atomic force microscopy
AFM
PCR
Fluorescence

Software Mentioned

MASCOT
GPS Explorer
BoxShade
TreeView
ClustalW
MaxQuant
Alignment
Multi Gauge

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