Downregulation of circ_0012152 inhibits proliferation and induces apoptosis in acute myeloid leukemia cells through the miR-625-5p/SOX12 axis.

Hematological Oncology
Zhen ShangYi Xiao

Abstract

Acute myeloid leukemia (AML) is a highly heterogeneous disease featured by a clonal proliferation derived from primitive hematopoietic stem/progenitor cells. Circular RNAs (circRNAs) have been identified as crucial regulators in the progression of various cancers, including AML. However, the molecular mechanism of AML is still not definite. This study aimed to explore the influences of circ_0012152 on cell development in AML cells and the underlying regulatory mechanism. The expression of circ_0012152, microRNA-625-5p (miR-625-5p) and sex-determining region Y-related high mobility group box 12 (SOX12) was detected by quantitative real-time polymerase chain reaction. The proliferation of AML cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay for cell viability, 5-ethynyl-2'-deoxyuridine incorporation assay for DNA biosynthesis and flow cytometry for cell cycle distribution, respectively. The death of AML cells was detected by flow cytometry. The protein expression was assessed by Western blot assay. Dual-luciferase reporter and RNA immunoprecipitation assays were carried out to examine the relationships among circ_0012152, miR-625-5p and SOX12. The expression of circ_0012152 was increased i...Continue Reading

References

Jun 10, 2005·Nature·Jun LuTodd R Golub
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefano VoliniaCarlo M Croce
Jul 13, 2007·The International Journal of Biochemistry & Cell Biology·Véronique LefebvreBhattaram Pallavi
Aug 7, 2009·The New England Journal of Medicine·Elaine R MardisTimothy J Ley
Oct 12, 2010·Methods in Molecular Biology·Julia Winter, Sven Diederichs
Jan 12, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Alan BurnettBob Löwenberg
Sep 19, 2016·Blood Reviews·Jeanette Prada-ArismendySarah Röthlisberger
Nov 20, 2016·British Journal of Haematology·Haixia WanHua Zhong
Aug 7, 2018·Lancet·Nicholas J ShortJorge E Cortes
Aug 22, 2018·BMC Cancer·Zikang ZhangHua Zhang
Sep 28, 2018·Advances in Experimental Medicine and Biology·Rupal OjhaVijay Kumar Prajapati
Dec 26, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Eric Chun Shen LeeMayuren Candasamy
Jan 11, 2019·Molecular Cancer·Qingfeng ShangShengfang Ge
May 28, 2019·Journal of Hematology & Oncology·Yan LiuLin Fu
Nov 30, 2019·Frontiers in Oncology·Muhammad JamalQiuping Zhang
Jan 1, 2020·Cancer Cell International·Zhaosheng ChenBin Liu
May 20, 2020·Cancer Biotherapy & Radiopharmaceuticals·Tao ShangWenbin Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

AML: Role of LSD1 by CRISPR (Keystone)

Find the latest rersearrch on the ability of CRISPR-Cas9 mutagenesis to profile the interactions between lysine-specific histone demethylase 1 (LSD1) and chemical inhibitors in the context of acute myeloid leukemia (AML) here.

Acute Myeloid Leukemia

Acute myeloid leukemia (AML) is a clinically and genetically heterogeneous disease with approximately 20,000 cases per year in the United States. AML also accounts for 15-20% of all childhood acute leukemias, while it is responsible for more than half of the leukemic deaths in these patients. Here is the latest research on this disease.

Acute Myeloid Leukaemia & RNA

Acute myeloid leukaemia (AML) is a common hematological type of cancer. As the population ages, there has been a rise in the frequency of AML. RNA expression has been used to see if there are different genetic profiles that exist within AML and whether these may underpin the variations in survival rates. Here is the latest research on AML and RNA.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.