Downregulation of ENDOCAN in myeloid leukemia cells inhibits proliferation and promotes apoptosis by suppressing nuclear factor‑κB activity

Molecular Medicine Reports
Lingling SunWei Yang

Abstract

Previous studies have demonstrated that ENDOCAN is elevated in leukemia, and it has been reported to be associated with poor prognosis. However, the functional role of ENDOCAN in the development of leukemia remains to be fully elucidated. In the present study, the expression levels of ENDOCAN were detected in THP‑1, U937, HL‑60 and K562 cells, and it was found that ENDOCAN was increased in U937 and K562 cells, compared with the other two cell lines. Subsequently, ENDOCAN was knocked down in U937 and K562 cells via lentiviral infection. It was found that cell proliferation and the expression of proliferating cell nuclear antigen were inhibited in myeloid leukemia cells following the silencing of ENDOCAN. ENDOCAN knockdown induced G0/G1‑phase cell cycle arrest in myeloid leukemia cells with a decreased expression of cyclin D1. Furthermore, cell apoptosis was increased in response to ENDOCAN silencing, which was accompanied by the downregulation of B‑cell lymphoma (BCL2) and the upregulation of BCL2‑associated X protein, cleaved caspases 3 and 9, and cleaved poly (ADP‑ribose) polymerase. Furthermore, it was demonstrated that the knockdown of ENDOCAN inhibited nuclear factor‑κB (NF‑κB) activity, as evidenced by the increased expres...Continue Reading

References

Aug 23, 1996·The Journal of Biological Chemistry·P LassalleA B Tonnel
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 28, 2005·Virchows Archiv : an International Journal of Pathology·Xavier DolcetXavier Matias-Guiu
Sep 9, 2005·Nature Reviews. Cancer·Yasuko KondoSeiji Kondo
Aug 11, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bogdan Dragos GrigoriuPhilippe Lassalle
Aug 2, 2007·Haematologica·Daniela CilloniGiuseppe Saglio
Feb 29, 2008·Journal of Toxicology and Environmental Health. Part a·Hsu-Huei WengChun-Yuh Yang
May 22, 2009·Journal of Neuropathology and Experimental Neurology·Claude-Alain MaurageMaryse Delehedde
Nov 3, 2009·Molecular and Cellular Endocrinology·Francesco Pacifico, Antonio Leonardi
Feb 13, 2010·Expert Opinion on Therapeutic Targets·Monica Lopez-Guerra, Dolors Colomer
Mar 22, 2012·Journal of Translational Medicine·Gabriele MadonnaPaolo Antonio Ascierto
Nov 2, 2013·Journal of the American Academy of Dermatology·Ilknur BaltaYasemin Gulcan Kurt
Mar 26, 2014·Journal of Cancer·Kazuaki OzakiMikihiro Tsutsumi
Apr 29, 2014·Microvascular Research·Dunja M MihajlovicTatjana N Cebovic
Jun 18, 2014·Molecular and Cellular Biochemistry·Zhe XuRuixiang Xia
Jul 12, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Wenyan ZhaoJingang Liu
Sep 15, 2014·Biotechnology Advances·Jianfeng GuoCaitriona M O'Driscoll
Nov 12, 2014·Current Medicinal Chemistry·Wei WangRuiwen Zhang
Dec 17, 2015·Canadian Journal of Gastroenterology & Hepatology·Nobuyuki ToshikuniMikihiro Tsutsumi
Jun 3, 2016·CA: a Cancer Journal for Clinicians·Kimberly D MillerAhmedin Jemal
Sep 21, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zhang SumeiWang Yuan
Dec 14, 2016·Blood·Patrick Auberger, Alexandre Puissant
May 26, 2017·International Journal of Cancer. Journal International Du Cancer·Xiaofeng WangLiping Su
Oct 13, 2017·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Onur BenderKadriye Serife Ugur

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Jan 6, 2021·Molecular and Cellular Biochemistry·Michele ScuruchiGiuseppe M Campo

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