Downregulation of lncRNA ANRIL suppresses growth and metastasis in human osteosarcoma cells

OncoTargets and Therapy
Hongya GuanYi-Sheng Wang

Abstract

This study was designed to research the potential function of lncRNA ANRIL in osteosarcoma (OS). Quantitative real-time PCR, cell counting kit-8, wound healing assay, Transwell assay, flow cytometric analysis, caspase activity analysis, and Western blot were carried out. ANRIL was remarkably upregulated in human OS tissues and cells, and knockdown of ANRIL significantly suppressed MG63 cell proliferation, migration, and invasion and promoted apoptosis. Moreover, our mechanistic research findings verified that ANRIL-influenced growth and apoptosis may be partly through regulation of caspase-3 and Bcl-2. Migration and invasion were influenced via ANRIL-mediated regulation of MTA1, TIMP-2, and E-cadherin. Our finding demonstrates that ANRIL plays vital roles in OS growth and metastasis.

Citations

Jan 14, 2021·International Journal of Molecular Sciences·Han Yeoung LeeJong Kook Park
Mar 22, 2021·Current Treatment Options in Oncology·Valeria A Ferretti, Ignacio E León
Jul 24, 2020·Anti-cancer Agents in Medicinal Chemistry·Onur TokgunHakan Akca
Oct 5, 2021·Journal of Biochemical and Molecular Toxicology·Weiguo MaQingxia Xu
Nov 13, 2021·Journal of Biochemical and Molecular Toxicology·Yueli ZhangRui Zhang

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Methods Mentioned

BETA
PCR
transfection
immunoprecipitation
protein assay

Software Mentioned

SPSS

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