Downregulation of lncRNA MALAT1 contributes to renal functional improvement after duodenal-jejunal bypass in a diabetic rat model

Journal of Physiology and Biochemistry
Dong WuSanyuan Hu

Abstract

Ameliorated renal function has been reported after bariatric surgery, but the mechanisms underlying this phenomenon are not well-studied. To investigate whether the long non-coding RNA (lncRNA) MALAT1 mediates the amelioration of diabetic nephropathy after duodenal-jejunal bypass (DJB) surgery, rats were assigned randomly into four groups: diabetic (DM) group, DM with DJB surgery group, DM with sham surgery group, and healthy control group. Food intake, body weight, oral glucose tolerance test (OGTT), urine albumin excretion rate (UAER), and glomerular filtration rate (GFR) were measured and histological examination of renal sections was performed. For in vitro study, HK-2 cells were cultured under various glucose concentrations following MALAT1 siRNA transfection. Expression levels of MALAT1, SAA3, IL-6, and TNF-α in rat renal tissues or HK-2 cell lines were evaluated by qRT-PCR and/or ELISA. Results showed DJB surgery improved the renal function of diabetic rats, as indicated by ameliorated UAER and GFR and attenuated glomerular hypertrophy. Expression of MALAT1 and its downstream target SAA3 was significantly downregulated in renal tissues after DJB, which in turn decreased the expression of the pro-inflammatory cytokines IL...Continue Reading

References

Sep 5, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G Frühbeck, J Gómez-Ambrosi
Jun 28, 2005·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·K SrinivasanP Ramarao
Feb 4, 2009·Nature Reviews. Genetics·Tim R MercerJohn S Mattick
Jan 7, 2010·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·H AshrafianT Athanasiou
May 4, 2011·Nature Reviews. Nephrology·Juan F Navarro-GonzálezJavier García-Pérez
Dec 6, 2012·Surgery for Obesity and Related Diseases : Official Journal of the American Society for Bariatric Surgery·Helen M HeneghanPhilip R Schauer
Mar 19, 2013·Cell·Pedro J Batista, Howard Y Chang
Jan 18, 2014·Investigative Ophthalmology & Visual Science·Biao YanQin Jiang
Apr 1, 2014·The New England Journal of Medicine·Philip R SchauerUNKNOWN STAMPEDE Investigators
Jun 11, 2014·JAMA : the Journal of the American Medical Association·Lars SjöströmLena M S Carlsson
Nov 21, 2014·Journal of Diabetes Investigation·Søs Skovsø
Dec 3, 2014·Cell Metabolism·Ippei ShimizuTohru Minamino
Mar 20, 2015·Journal of Cellular and Molecular Medicine·Prasanth PuthanveetilSubrata Chakrabarti
Jun 10, 2015·Nature Reviews. Endocrinology·Gema Frühbeck
Feb 13, 2016·Lancet·George A BrayJohn P H Wilding
Feb 19, 2016·Current Opinion in Nephrology and Hypertension·Yuliya LytvynDavid Z I Cherney
Feb 28, 2016·Current Opinion in Pharmacology·Laura Denby, Andrew H Baker
Mar 8, 2016·Surgery for Obesity and Related Diseases : Official Journal of the American Society for Bariatric Surgery·Sikarin UpalaAnawin Sanguankeo
Apr 29, 2016·International Journal of Obesity : Journal of the International Association for the Study of Obesity·W GuoS Hu
May 4, 2016·Nature Reviews. Nephrology·Johan M Lorenzen, Thomas Thum
Aug 10, 2016·Trends in Endocrinology and Metabolism : TEM·Rafael MoncadaGema Frühbeck
Nov 21, 2016·Surgery for Obesity and Related Diseases : Official Journal of the American Society for Bariatric Surgery·Tingfeng WangBo Yu
Jan 17, 2017·Nature Reviews. Nephrology·Niels Olsen Saraiva CâmaraKumar Sharma
Apr 2, 2017·Cytokine & Growth Factor Reviews·Eva Feigerlová, Shyue-Fang Battaglia-Hsu

❮ Previous
Next ❯

Citations

Dec 20, 2019·IUBMB Life·Youyou YanChunli Song
Aug 12, 2020·Nature Reviews. Nephrology·Neil G Docherty, Carel W le Roux
Apr 21, 2020·Journal of Alzheimer's Disease : JAD·Murali Vijayan, P Hemachandra Reddy
Jul 14, 2020·Journal of Diabetes Research·Jiarong LvShizhong Bu
Feb 8, 2019·Current Diabetes Reports·Lixia XuZhen Chen

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
enzyme-linked immunosorbent assay
ELISA
transfection
PCR

Software Mentioned

Image
GraphPad Prism
Pro Plus

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.