Downregulation of Long Non-coding RNA Nuclear Paraspeckle Assembly Transcript 1 Inhibits MEG-01 Differentiation and Platelet-Like Particles Activity

Frontiers in Genetics
Weihua BianChunxiang Zhang

Abstract

Platelets are derived from megakaryocytes and play an important role in blood coagulation. By using high throughput sequencing, we have found that the long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) is abundant in platelets (GEO ID: 200097348). However, little is known about its role in regulating megakaryocyte differentiation and platelet activity. This study aims to clarify the effect of NEAT1 on MEG-01 differentiation and platelet-like particle (PLP) activity. NEAT1 in MEG-01 cells was knocked down by siRNA transfection. The adhesion of MEG-01 and PLP to collagen-coated coverslips was observed under a fluorescence microscope. Flow cytometry was used to investigate cell apoptosis, cell cycle, the levels of D41/CD42b on MEG-01 cells and CD62P on PLPs. Quantitative real-time polymerase chain reaction was used to detect NEAT1 and IL-8 expression levels. Western blot was used to measure the protein levels of Bcl-2, Bax, cleaved caspase-3, and IL-8. RNA-binding protein immunoprecipitation was used to detect the interaction of NEAT1 and splicing factor proline/glutamine-rich (SFPQ). Results showed that NEAT1 knockdown decreased the adhesion ability of thrombin-stimulated MEG-01 and PLP. The expression...Continue Reading

References

Feb 20, 2003·The Journal of Cell Biology·Murray C H ClarkeSimon B Brown
Aug 19, 2007·BMC Developmental Biology·Sigrid A LehnertPaul L Greenwood
Jun 23, 2015·Development, Growth & Differentiation·Chenghai WangDuonan Yu
Apr 12, 2016·BioMed Research International·Xiao-Lei YangAi-Ping Yu
Aug 27, 2016·Scientific Reports·Janette Bester, Etheresia Pretorius
Apr 6, 2017·Journal of Cellular Biochemistry·Qiyuan WangZeling Long
Aug 2, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Krzysztof M KrawczykMartin E Johansson
Aug 11, 2017·Nature Communications·Adrian SchwarzerJan-Henning Klusmann
Jan 10, 2018·Oncotarget·Noam FallikRoi Gazit
Jun 25, 2018·The American Journal of Emergency Medicine·Qinghe HuangRongfang Zhang
Jul 18, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Aijun YangJianzhong Xu
Aug 9, 2018·Frontiers in Cell and Developmental Biology·Emil H ErnstKarin Lykke-Hartmann
Dec 15, 2018·Cell·Anna HupalowskaMagdalena Zernicka-Goetz
Dec 24, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-Fard, Mohammad Taheri
Feb 6, 2019·International Journal of Molecular Sciences·Felix PrinzChristiane Klec
May 13, 2019·Molecular Immunology·Swati DahariyaRavi Kumar Gutti
Jun 28, 2019·Cell Death & Disease·Shanshan WangBo Zuo
Jul 25, 2019·Molecular & Cellular Proteomics : MCP·Anja M BillingJohannes Graumann
Dec 14, 2019·Cell Stress·Haiyan An, Tatyana A Shelkovnikova
Feb 6, 2020·International Journal of Molecular Sciences·Bernadett SzilágyiBéla Nagy
Feb 20, 2020·Non-coding RNA Research·Gabriel Pisani, Byron Baron
Feb 23, 2020·Frontiers in Genetics·Lihong PengLiqian Zhou
Mar 3, 2020·Blood Advances·Christian EscobarEleftherios T Papoutsakis
Mar 17, 2020·Critical Care Clinics·Tom van der Poll, Robert I Parker
Mar 18, 2020·Blood Cells, Molecules & Diseases·Ming-Yue DuanNian-Cai Peng
Mar 18, 2020·Science Advances·Helena G AsenjoDavid Landeira
Oct 1, 2019·Molecular & Cellular Proteomics : MCP·Anja M BillingJohannes Graumann

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Datasets Mentioned

BETA
ID
200097348

Methods Mentioned

BETA
transfection
flow cytometry
Assay
immunoprecipitation assay
Protein Assay
Immunoprecipitation
RIP

Software Mentioned

ImageJ

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Related Papers

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Yan ZhangYan Guo
BioRxiv : the Preprint Server for Biology
Michail S KukharskyTatyana A Shelkovnikova
Cellular and Molecular Life Sciences : CMLS
Ziqiang WangWeiren Huang
© 2021 Meta ULC. All rights reserved