Downregulation of long noncoding RNA Sox2ot protects PC-12 cells from hydrogen peroxide-induced injury in spinal cord injury via regulating the miR-211-myeloid cell leukemia-1 isoform2 axis

Journal of Cellular Biochemistry
Dong YinYunbing Chang

Abstract

This study aimed to investigate the effects and possible mechanisms of long noncoding RNA (lncRNA) Sox2 overlapping transcript (Sox2ot) on hydrogen peroxide (H2 O2 )-induced injury in pheochromocytoma (PC-12) cells. PC-12 cells were treated with H2 O2 to cell injury. The cells were transfected with short-hairpin RNA directed against Sox2ot (sh-Sox2ot), small interfering RNA directed against myeloid cell leukemia-1 (MCL-1) isoform2 (si-MCL-1), a miR-211 mimic, a miR-211 inhibitor, and their negative controls. Under different transfected treatments, cell viability, migration, invasion, and apoptosis as well as the expressions of apoptosis- and autophagy-related proteins were investigated. Besides, the regulatory relationships between Sox2ot and miR-211, miR-211 and MCL-1, as well as between MCL-1 and the protein kinase B (Akt)/mammalian target of the rapamycin (mTOR)/p70 ribosomal S6 protein kinase (p70S6K) signaling pathway were explored. Suppression of Sox2ot inhibited H2 O2 -induced PC-12 cell injury by increasing cell viability, migration, invasion, and decreasing apoptosis and autophagy. Moreover, suppression of Sox2ot increased miR-211 expression and alleviated H2 O2 -induced injury in PC-12 cells possibly via upregulation ...Continue Reading

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Citations

Oct 23, 2019·Journal of Cellular Physiology·Tao ChenLei Guo
Nov 19, 2019·BioMed Research International·Fei WangZhenxing Li
Jul 14, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Xiaohong YangJing Yang
May 7, 2021·Regenerative Medicine·Xu-Dong GuoXue-Wen Kang
Jul 30, 2021·Journal of Orthopaedic Surgery·Yiguang Zhou, Feng Yu
Oct 19, 2021·Experimental and Therapeutic Medicine·Duoping ChenRui Lv

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