Downregulation of microRNA-448 inhibits IL-1β-induced cartilage degradation in human chondrocytes via upregulation of matrilin-3

Cellular & Molecular Biology Letters
Hao YangYongjun Shang

Abstract

Osteoarthritis is characterized by the continuous degradation of the articular cartilage. The microRNA miR-448 has been found to be broadly involved in cellular processes, including proliferation, apoptosis, invasion and EMT. While aberrant expression of miR-448 has been found in multiple cancers, its level in osteoarthritis cartilage and its role in the progression of this disease are still unknown. Here, we examined the functional roles of miR-448 and its expression in osteoarthritis tissues, including IL-1β-stimulated osteoarthritis chondrocytes. Chondrocytes were isolated from human articular cartilage and stimulated with IL-1β. The expression levels of miR-448 in the cartilage and chondrocytes were both determined. After transfection with an miR-448 mimic or inhibitor, the mRNA levels of aggrecan, type II collagen and MMP-13 were determined. Luciferase reporter assay, qRT-PCR and western blot were performed to explore whether matrilin-3 was a target of miR-448. Furthermore, we co-transfected chondrocytes with miR-448 inhibitor and siRNA for matrilin-3 and then stimulated them with IL-1β to determine whether miR-448-mediated IL-1β-induced cartilage matrix degradation resulted from directly targeting matrilin-3. The level of...Continue Reading

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Citations

Dec 7, 2019·Experimental and Therapeutic Medicine·Yunchao ChuXin Wang
Jun 11, 2019·Molecular Medicine Reports·Hui ZhuYuping Tang
Dec 24, 2019·Cellular & Molecular Biology Letters·Rafal Bartoszewski, Aleksander F Sikorski
Mar 4, 2020·Cellular & Molecular Biology Letters·Hongda DingChaoliu Dai
Sep 27, 2018·Cellular & Molecular Biology Letters·Rafal Bartoszewski, Aleksander F Sikorski
Jun 25, 2021·Journal of Clinical Laboratory Analysis·Chengyun WangHuirong Lin
Jan 12, 2020·Wiley Interdisciplinary Reviews. RNA·Siyu JiangMin Yang

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Methods Mentioned

BETA
transfection
ELISA
PCR

Software Mentioned

Image J
GraphPad
GraphPad Prism

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