Downregulation of microRNA‑198 suppresses cell proliferation and invasion in retinoblastoma by directly targeting PTEN

Molecular Medicine Reports
Dongdong WeiYingli Wang

Abstract

A number of studies have highlighted that aberrantly expressed microRNAs (miRNAs/miRs) serve crucial roles in the tumorigenesis and tumor development of retinoblastoma (RB). Hence, a full investigation of the biological roles and regulatory mechanisms of miRNAs in RB may provide novel therapeutic targets for patients with this malignancy. miR‑198 is frequently abnormally expressed in various types of human cancers. However, the expression level, biological roles and underlying mechanisms of miR‑198 in RB remain to be elucidated. In the present study, miR‑198 expression was upregulated in RB tissues and cell lines. Silencing of miR‑198 attenuated cell proliferation and invasion in RB. In addition, phosphatase and tensin homolog deleted on chromosome ten (PTEN) was predicted as a potential target of miR‑198 using bioinformatics analysis. Subsequent luciferase reporter assay indicated that the 3'‑untranslated region of PTEN can be directly targeted by miR‑198. Furthermore, miR‑198 inhibition increased the PTEN expression at the mRNA and protein levels in RB cells. In addition, PTEN mRNA expression was downregulated in RB tissues, and this downregulation was inversely associated with the expression level of miR‑198. PTEN knockdown ...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 28, 2002·Current Biology : CB·Eric G Moss
Apr 16, 2002·European Journal of Gastroenterology & Hepatology·Guo FeiPeter Malfertheiner
Aug 27, 2005·Urologia Internationalis·Ismail Turker KoksalSalih Sanlioglu
Aug 29, 2006·Oncogene·A BalmerF Munier
Nov 24, 2006·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Jing TaoChunyou Wang
Nov 3, 2010·Nucleic Acids Research·Ana Kozomara, Sam Griffiths-Jones
Mar 15, 2012·Lancet·Helen DimarasBrenda L Gallie
Jul 17, 2012·Journal of Neurosurgery. Pediatrics·Pascal JabbourCarol Shields
Oct 17, 2012·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Lin YeXishuang Song
Nov 2, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Bo QiHan-Chen Li
Jul 9, 2014·Revista brasileira de ginecologia e obstetrícia : revista da Federação Brasileira das Sociedades de Ginecologia e Obstetrícia·Luciano Fernandes LouresAgnaldo Lopes da Silva-Filho
Aug 1, 2015·International Journal of Molecular Sciences·Jian WangZhongmin Zou
Nov 11, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shujun WuGuoqiang Zhao
Mar 13, 2016·Biochemical and Biophysical Research Communications·Shilian ZhangLijie Wang
Oct 30, 2016·Anticancer Research·Barbara D'AngeloAntonio Giordano
Dec 19, 2016·International Journal of Ophthalmology·Li-Lun WangYan-Qin Feng
Apr 21, 2017·Journal of Neuro-oncology·Er NieYongping You
Jun 29, 2017·Journal of Cellular Physiology·Khodayar GolabchiHamed Mirzaei
Oct 1, 2017·Experimental and Therapeutic Medicine·Yong LiangZhu Liang
Nov 16, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wen-Wei Zou, Su-Ping Xu

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Citations

Oct 24, 2018·Journal of Cancer Research and Clinical Oncology·Lara Elis Alberici DelsinMaria Sol Brassesco

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