Downregulation of microRNA‑33a promotes cyclin‑dependent kinase 6, cyclin D1 and PIM1 expression and gastric cancer cell proliferation

Molecular Medicine Reports
Yudong WangWei Liu

Abstract

Although microRNA‑33 (miR‑33) family members are known to be involved in the regulation and balancing of cholesterol metabolism, fatty acid oxidation and insulin signaling, their functions in carcinogenesis are controversial and the underlying mechanisms have remained elusive. Gastric cancer is the fourth most common malignancy in the world; however, the dysregulation and function of miR‑33 family members in gastric cancer have not been extensively studied. The present study reported that a miR‑33 family member, miR‑33a, was significantly downregulated in gastric cancer tissues and gastric cancer cell lines. Of note, the expression of miR‑33a was inversely correlated with pathological differentiation and metastasis as well as gastric cancer biomarker CA199. A cell‑counting kit‑8 assay showed that transfection of the SGC‑7901 gastric cell line with miR‑33a‑overexpression plasmid inhibited the capability of the cells to proliferate. Furthermore, overexpression of miR‑33a led to cell cycle arrest of SGC‑7901 cells in G1 phase. In addition, a luciferase reporter assay showed that miR‑33a directly targeted cyclin‑dependent kinase 6 (CDK6), cyclin D1 (CCND1) and serine/threonine kinase PIM‑1. In gastric cancer specimens, the reduced ...Continue Reading

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Citations

Apr 4, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Di-Di ChenYing-Peng Huang
Sep 28, 2017·Oncotarget·Omer Faruk KaratasMichael Ittmann
Nov 3, 2017·Scientific Reports·Po-Shuan HuangKwang-Huei Lin
Aug 28, 2021·Journal for Immunotherapy of Cancer·Rongmu XiaYanjun Mi

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