Downregulation of miR-221, -30d, and -15a contributes to pathogenesis of prostate cancer by targeting Bmi-1.

Biochemistry. Biokhimii︠a︡
Hanqing XuanYiran Huang

Abstract

Prostate cancer is the second leading cause of cancer-related deaths of men. Bmi-1, a member of PcG family of proteins, has been implicated in the pathogenesis of prostate cancer, and disturbed profile of microRNAs (miRNAs) has been found in prostate cancer tissues. How Bmi-1 is regulated by miRNAs is unclear. In this study, we screened 18 miRNAs that potentially repress the expression of Bmi-1 using a dual luciferase system and found that 12 miRNAs could bind with the 3'-untranslated region of Bmi-1 mRNA. Using qRT-PCR, we found that expression of miR-221, -15a, and -30d was significantly reduced in prostate cancer tissues. Subsequent functional study indicated that miR-221 and miR-30d can repress prostate cancer cell proliferation, and this effect can be partially rescued by Bmi-1 overexpression. Our study constructs the relation between downregulated miR-221 and miR-30d and prostate cancer pathogenesis. These results indicate that miR-221 and miR-30d are candidate tumor suppressor miRNAs in prostate cancer and therefore serve as potential clinical classification markers and therapeutic targets for human prostate cancer.

References

Mar 6, 2009·Nature Reviews. Cancer·Milena S NicolosoGeorge A Calin
Nov 12, 2009·Cancer Research·Resham BhattacharyaPriyabrata Mukherjee
Jan 28, 2014·Journal of Cellular Biochemistry·Xiao-Fei QiangLi-Hua Shi
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Citations

Jun 8, 2015·Endocrine·Mariarosaria BoccellinoMichele Caraglia
Jun 10, 2016·Journal of Human Genetics·Satoko KojimaYukio Naya
Jun 28, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Rui ZhangJinghui Bai
Aug 24, 2019·Clinical and Experimental Medicine·Yulian MytsykVictor Dosenko
Jan 25, 2019·Oncology Letters·Minglei ZhangHongyan Li
Jan 22, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Zhiwei MaMingxing Qiu
Apr 26, 2017·International Journal of Molecular Medicine·Likun YanJianfeng Yao

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