Downregulation of miR-221 Inhibits Cell Migration and Invasion through Targeting Methyl-CpG Binding Domain Protein 2 in Human Oral Squamous Cell Carcinoma Cells

BioMed Research International
Shuqi HeYu Chen

Abstract

Oral squamous cell carcinoma (OSCC), the most frequent of all oral cancers, is a type of highly malignant tumors with a high capacity to invade locally and form distant metastases. An increasing number of studies have shown that microRNAs (miRNAs) play an important role in regulating cancer metastasis and invasion. In the present study, we detected the expression of miR-221 in two highly metastatic OSCC cell lines and two OSCC cell lines that are less metastatic using quantitative real-time PCR analysis (qRT-PCR). The qRT-PCR results indicate that miR-221 is upregulated in highly metastatic OSCC cell lines. Then, miR-221 expression was knocked down by transfection with miR-221 inhibitor, and UM1 cell migration and invasion were assessed using transwell migration and invasion assays. The results indicate that inhibition of miR-221 suppressed migration and invasion of UM1 cells. Furthermore, methyl-CpG binding domain protein 2 (MBD2) was identified as a direct target gene of miR-221. Additionally, MBD2 silencing could partly reverse the effect of miR-221 on cell migration and invasion. In conclusion, downregulation of miR-221 inhibits cell migration and invasion at least partially through targeting MBD2 in the human OSCC cell lin...Continue Reading

References

May 8, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Pouya PakneshanShafaat A Rabbani
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Sep 17, 2004·Nature·Victor Ambros
Mar 3, 2006·Genes & Development·Marco Antonio Valencia-SanchezRoy Parker
Oct 31, 2006·Oral Oncology·Amanajás Francisco Carlos de AguiarOslei Paes de Almeida
Dec 22, 2007·Journal of Dental Research·S Choi, J N Myers
Jan 11, 2008·Nature·Sohail F TavazoieJoan Massagué
Mar 4, 2009·Nature Cell Biology·Julia WinterSven Diederichs
Dec 2, 2009·Cell Adhesion & Migration·Lin Mei, Wen-Cheng Xiong
Dec 31, 2009·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Avraham ZiniHarold D Sgan-Cohen
Jan 14, 2011·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Chun-Ju YangShu-Chun Lin
Jul 13, 2011·Cancer Research·Barbara StefanskaMoshe Szyf
Nov 16, 2011·Current Molecular Medicine·M GarofaloG Condorelli
Dec 18, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Thaís Brilhante PontesMarília Arruda Cardoso Smith
Mar 4, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Fan YangTao Wang
Jul 7, 2015·Life Sciences·Weifeng WangChangjun Lin
Jul 8, 2015·Biochimica Et Biophysica Acta·Julia Liz, Manel Esteller
Jul 24, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Heba W Z KhellaGeorge M Yousef
Aug 1, 2015·Cancers·Esteban A Orellana, Andrea L Kasinski

❮ Previous
Next ❯

Citations

Jan 10, 2017·Clinics and Research in Hepatology and Gastroenterology·Jing ZhaoKai Wang
Jul 13, 2017·Tissue Engineering. Part a·Anthony MonteforteAaron B Baker
Aug 7, 2017·Journal of Cellular and Molecular Medicine·Kai WangHongfeng Zhai
Feb 3, 2018·International Journal of Oncology·Hiroki NagaiHiroki Bukawa
Jul 3, 2021·International Journal of Molecular Sciences·Adriana FodorAngela Cozma

❮ Previous
Next ❯

Methods Mentioned

BETA
Transfection
PCR
reverse transcription PCR
Protein Assay
Assay

Software Mentioned

Image Pro Plus
SPSS
Targetscan
miRanda

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

International Journal of Clinical and Experimental Pathology
Jiang-Tao ChenJun-Jie Zhang
International Journal of Clinical and Experimental Medicine
Ya-Jun ZhangLei Li
International Journal of Clinical and Experimental Pathology
Guang-Jian LuChen-Guang Zhang
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Wei DingLan Wang
© 2022 Meta ULC. All rights reserved