Downregulation of miR-224 and let-7i contribute to cell survival and chemoresistance in chronic myeloid leukemia cells by regulating ST3GAL IV expression

Gene
Huimin ZhouLi Jia

Abstract

Acquired resistance to imatinib is frequently associated with poor clinical outcome of chronic myeloid leukemia (CML) patient. To date, evidence indicates that protein glycosylation and its upstream regulators might be implicated in tumorigenesis and chemoresistance occurrence. In current study we initially explored N-glycan profiles on the surface of CML cell lines and bone marrow mononuclear cells (BMMC) of CML patients by using mass spectrometry (MS) analysis. An elevated sialylation was detected in K562R cells (CML cells with imatinib resistance phenotype) compare to K562 cells. By quantitative real time-PCR (qRT-PCR) and western blotting analysis we observed that imatinib resistant K562R cells exhibited marked high levels of CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase (ST3Gal IV) as compared to imatinib sensitive K562 cells. Further studies revealed that manipulated expression of ST3GAL IV led to the significant alterations of cell cycle distribution, apoptotic signal, cell proliferation and the effectiveness of imatinib treatment. Using microRNA array, miRNA database searching and luciferase reporter assay, we identified that miR-224 and let-7i directly regulate the expression of ST3GAL IV gene....Continue Reading

Citations

May 14, 2019·Journal of Cellular Biochemistry·Hossein SharifiMohsen Taghizadeh
Sep 14, 2018·Frontiers in Oncology·Fabio CorràStefano Volinia
Dec 8, 2019·Journal of Clinical Medicine·Stasė GasiulėGiedrius Vilkaitis
Sep 22, 2018·Frontiers in Oncology·Xingchen PangXiang Li
Oct 17, 2018·International Journal of Molecular Sciences·Mirco Di MarcoRosa Visone
Jan 7, 2021·Omics : a Journal of Integrative Biology·Medi KoriKazim Yalcin Arga
Feb 4, 2021·Experimental Hematology & Oncology·Daniel T JohnsonDong-Er Zhang
Nov 2, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Desmond O AcheampongFoster Kyei
Mar 25, 2021·Seminars in Cancer Biology·Sara Peixoto da SilvaM Helena Vasconcelos

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