Downregulation of miR-26b-5p, miR-204-5p, and miR-497-3p Expression Facilitates Exercise-Induced Physiological Cardiac Hypertrophy by Augmenting Autophagy in Rats

Frontiers in Genetics
Jie QiJun Zhang

Abstract

Exercise-induced autophagy is associated with physiological left ventricular hypertrophy (LVH), and a growing body of evidence suggests that microRNAs (miRNAs) can regulate autophagy-related genes. However, the precise role of miRNAs in exercise induced autophagy in physiological LVH has not been fully defined. In this study, we investigated the microRNA-autophagy axis in physiological LVH and deciphered the underlying mechanism using a rat swimming exercise model. Rats were assigned to sedentary control (CON) and swimming exercise (EX) groups; those in the latter group completed a 10-week swimming exercise without any load. For in vitro studies, H9C2 cardiomyocyte cell line was stimulated with IGF-1 for hypertrophy. We found a significant increase in autophagy activity in the hearts of rats with exercise-induced physiological hypertrophy, and miRNAs showed a high score in the pathway enriched in autophagy. Moreover, the expression levels of miR-26b-5p, miR-204-5p, and miR-497-3p showed an obvious increase in rat hearts. Adenovirus-mediated overexpression of miR-26b-5p, miR-204-5p, and miR-497-3p markedly attenuated IGF-1-induced hypertrophy in H9C2 cells by suppressing autophagy. Furthermore, miR-26b-5p, miR-204-5p, and miR-49...Continue Reading

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Citations

Aug 28, 2020·Frontiers in Pharmacology·Kefa XiangXia Liu
Dec 5, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Zhen SunZhongqun Wang
Feb 3, 2021·Autophagy·Soudeh Ghafouri-FardMohammad Taheri
Dec 18, 2020·Journal of Sport and Health Science·Guiling WuFeng Gao
Mar 30, 2021·Oxidative Medicine and Cellular Longevity·Mingjing YanJian Li

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Datasets Mentioned

BETA
GSE143935

Methods Mentioned

BETA
light microscopy
PCR
electrophoresis
electron microscopy

Software Mentioned

ImageJ
TargetScan
ScopePhoto
GenePix Pro
Pro Plus
GraphPad Prism
Image
DNAMAN
Prism

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