Downregulation of miR-432 activates Wnt/β-catenin signaling and promotes human hepatocellular carcinoma proliferation

Oncotarget
Nan JiangGuo-Ying Wang

Abstract

Sustained cell growth and proliferation, one of the hallmarks of cancer, is considered to responsible for cancer-related deaths by disorganizing the balance of growth promotion and growth limitation. Aberrant activation of the Wnt/β-catenin signaling pathway leads to cell proliferation, growth and survival, and promotes the development of various human tumors, including hepatocellular carcinoma. Elucidating the molecular mechanism of this abnormality in hepatocellular carcinoma carcinogenesis may improve diagnostic and therapeutic strategies for this malignancy. Herein, we report that the expression of miR-432 was markedly downregulated in hepatocellular carcinoma cell lines and tissues, and upregulation of miR-432 inhibited, whereas downregulation of miR-432 enhanced the proliferation and tumorigenicity of hepatocellular carcinoma cells both in vitro and in vivo. Furthermore, miR-432 directly targeted and suppressed multiple regulators of the Wnt/β-catenin signaling cascade, including LRP6, TRIM29 and Pygo2, which subsequently deactivated Wnt/β-catenin signaling pathway. Finally, miR-432 expression was inversely correlated with three targets in clinical hepatocellular carcinoma samples. These results demonstrated that miR-432 ...Continue Reading

References

Feb 9, 2000·Japanese Journal of Cancer Research : Gann·Y KondoS Hirohashi
May 4, 2002·Nature Cell Biology·Barry ThompsonMariann Bienz
Mar 15, 2003·Developmental Cell·Nicholas S TolwinskiEric Wieschaus
Sep 17, 2004·Nature Reviews. Genetics·Randall T MoonAjamete Kaykas
May 4, 2005·Cancer Research·Richard I Gregory, Ramin Shiekhattar
Aug 16, 2005·Journal of Hepatology·Philippe MerleJack R Wands
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Jun 8, 2006·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Lesleyann HawthornHeinz Baumann
Oct 25, 2006·Nature Reviews. Cancer·George A Calin, Carlo M Croce
Nov 23, 2006·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Oleg K GlebovIlan R Kirsch
Dec 2, 2006·Nature Reviews. Drug Discovery·Nick Barker, Hans Clevers
Jun 29, 2007·Annals of Surgical Oncology·Yoshimasa KosakaMasaki Mori
Feb 19, 2008·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Britta SkawranLudwig Wilkens
Mar 6, 2010·Journal of Neuro-oncology·Zhan-Xiang WangHai-Dong Wang
Feb 26, 2011·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayDonald Maxwell Parkin
May 9, 2012·The Journal of Clinical Endocrinology and Metabolism·Daniela D'AngeloAlfredo Fusco
Jun 12, 2012·Cell·Hans Clevers, Roel Nusse
Jun 4, 2013·The Journal of Clinical Investigation·Yael NemlichGal Markel

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Citations

Mar 20, 2018·Intractable & Rare Diseases Research·Naixiang ZhaiJinxiang Han
May 28, 2019·Journal of Cellular Physiology·Xinqi HuangZhihe Zhao
May 15, 2020·Cancer Biotherapy & Radiopharmaceuticals·Chunhui DongYanchao Wang
Nov 12, 2015·Pharmacogenomics·Celestino SarduRaffaele Marfella
Jan 3, 2020·International Journal of Molecular Medicine·Xudong ChenMeixiao Zhu
Jul 7, 2018·Frontiers in Pharmacology·Xiaobo NieYan-Dong Wang
Dec 20, 2018·Acta Biochimica Et Biophysica Sinica·Mingxing XuRuiyun Xu
Dec 17, 2020·Cancers·Tomasz M GrzywaPaweł K Włodarski
May 3, 2019·Respiratory Medicine·Katherine FesenRoopa Siddaiah
Apr 24, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Feng LiLu Bai

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Methods Mentioned

BETA
PCR
flow cytometry
transfection
nuclear translocation
Assay
antisense oligonucleotide

Software Mentioned

SPSS13
TargetScan
miRanda
SPSS

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