Downregulation of miR-522 suppresses proliferation and metastasis of non-small cell lung cancer cells by directly targeting DENN/MADD domain containing 2D

Scientific Reports
Tianze ZhangLinyou Zhang

Abstract

Non-small cell lung cancer (NSCLC), one of the most common causes of cancer-related death, is a worldwide public health problem. MicroRNAs (miRNAs) have recently been identified as a novel class of regulators of carcinogenesis and tumor progression, including miRNAs associated with NSCLC. This study aimed to explore the role of miR-522 in NSCLC and the mechanisms underlying this role. We report here that miR-522 expression was significantly increased in both human NSCLC tissues and cell lines. Furthermore, an MTT assay, 5-Ethynyl-2'-deoxyuridine (EdU) assay kit and flow cytometry confirmed that the inhibition of miR-522 suppressed NSCLC cells proliferation and induced apoptosis. Compared with miR-522 overexpression, miR-522 inhibitor markedly reduced cells migration and invasion, as indicated by wound-healing and transwell assays. In addition, a luciferase assay identified DENN/MADD domain containing 2D (DENND2D) as a direct target of miR-522. qRT-PCR and western blot analyses indicated the reciprocal expression of miR-522 and DENND2D in NSCLC tissue samples. DENND2D was involved in miR-522 induced proliferation and metastasis of NSCLC cells by a miRNA-masking antisense oligonucleotides (miR-mask) technology. These data highlig...Continue Reading

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Citations

Jul 5, 2019·The Kaohsiung Journal of Medical Sciences·Hong-Chang ZhangChang-Bao Chen
Nov 20, 2019·Expert Opinion on Drug Discovery·Kenneth K W ToWilliam C S Cho
Jan 3, 2020·Journal of Cellular and Molecular Medicine·Xiaozhen LiuCaicun Zhou
Jun 21, 2020·Translational Psychiatry·Paula RoviraMarta Ribasés
Jun 4, 2019·Frontiers in Pharmacology·Svetlana Miroshnichenko, Olga Patutina
Apr 21, 2020·Current Pharmaceutical Biotechnology·Atieh Hashemi, Gilar Gorji-Bahri
Aug 13, 2021·Cell Death Discovery·Yan LiuZuozhang Yang

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Methods Mentioned

BETA
GTPases
nucleotide exchange
transfection
flow cytometry
PCR
Infrared Imaging
Assay

Software Mentioned

TargetScan
miRanda

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