Downregulation of miR‑135a predicts poor prognosis in acute myeloid leukemia and regulates leukemia progression via modulating HOXA10 expression

Molecular Medicine Reports
Hongwei Xu, Quan Wen

Abstract

MicroRNA‑135a (miR‑135a) has been shown to exert important roles in various human cancer types, such as glioblastoma, thyroid carcinoma and renal carcinoma. However, the function of miR‑135a in acute myeloid leukemia (AML) remains largely unknown. In the present study, it was demonstrated that miR‑135a expression was significantly downregulated in AML cells compared with normal control cells. Furthermore, the downregulation of miR‑135a in patients with AML predicted poor prognosis. Through functional experiments, overexpression of miR‑135a was demonstrated to significantly inhibit the proliferation and cell cycle of AML cells, while it promoted cellular apoptosis. miR‑135a directly targeted HOXA10 in AML cells. miR‑135a overexpression significantly suppressed the mRNA and protein levels of HOXA10 in AML cells. Moreover, there was an inverse association between miR‑135a expression and HOXA10 level in AML samples. Additionally, by ectopic expression of HOXA10, restoration of HOXA10 significantly abolished the effects of miR‑135a overexpression on AML cell proliferation, cell cycle and apoptosis. In conclusion, the present study demonstrated that miR‑135a serves as a tumor suppressor in AML by targeting HOXA10, and miR‑135a may be...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Emmanuelle PasseguéIrving L Weissman
Nov 22, 2008·The Journal of Biological Chemistry·Hao WangElizabeth A Eklund
Nov 4, 2010·Blood·Guido MarcucciClara D Bloomfield
Aug 30, 2011·Nature Medicine·Kolja EppertJohn E Dick
Apr 12, 2012·The Journal of Biological Chemistry·Chirag A ShahElizabeth A Eklund
Mar 26, 2013·American Journal of Hematology·Elihu H Estey
Mar 13, 2014·Cell Stem Cell·Antonija Kreso, John E Dick
Jun 22, 2014·Cell Biochemistry and Biophysics·Baoxiu LiGuolong Liu
Dec 6, 2014·Frontiers in Genetics·Mona KhalajChristopher Y Park
May 12, 2016·Digestive Diseases and Sciences·Shangfeng SunYanfeng Lv
Jun 28, 2016·Journal of Integrative Neuroscience·Ricardo Zavala-YoeLuz M Cordero
Oct 6, 2016·Oncotarget·Qiong LiaoGuosheng Jiang
Oct 16, 2016·Journal of Cancer Research and Clinical Oncology·Xiang WangRongrong Zhang
Mar 21, 2017·Oncology Research·Xiaobo ZhangXiaodong Tan
Mar 28, 2017·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Xiao-Han XuMing-Hui Duan
May 10, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Liru LiuKuisheng Chen
Sep 5, 2017·Cancer Biomarkers : Section a of Disease Markers·Xue ZhaoLinghua Zhang
Sep 5, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yaoyao XiongLin Qi
Sep 6, 2017·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·M KangJ Shi
Nov 16, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Suwei ZhangJun Yin
Dec 23, 2017·Cell Communication and Signaling : CCS·Daniela M Gomez ZubietaKalyan C Kondapalli
Feb 10, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Peng YeXinghua Long

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Citations

Jul 3, 2021·Cancers·Ezgi Ozyerli-Goknar, Tugba Bagci-Onder

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