Downregulation of neutral invertase activity in sugarcane cell suspension cultures leads to a reduction in respiration and growth and an increase in sucrose accumulation

Functional Plant Biology : FPB
Debra RossouwJan-Hendrik Groenewald

Abstract

Suspension cultures were used as a model system to investigate sucrose metabolism in four sugarcane (Saccharum spp. interspecific hybrids) cell lines transformed with antisense neutral invertase (NI) constructs. Throughout a 14-day growth cycle two cell lines in which the antisense sequence was under the control of a tandem CaMV-35S: maize ubiquitin promoter showed a strong reduction in NI activity, as well as reduced hexose and increased sucrose concentrations in comparison to the control line. In lines where the antisense NI sequence was under the control of the weaker CaMV-35S promoter alone, changes in enzyme activity and sugar concentrations were intermediate to those of the more strongly inhibited lines and the control. In comparison to the control line, a higher sucrose to hexose ratio, i.e. increased purity, was obtained in all the lines with reduced NI activity. The in vivo rate of sucrose hydrolysis was reduced in the transgenic lines, suggesting a concomitant reduction in the flux through the 'futile cycle' of sucrose breakdown and re-synthesis. Differences between the transgenic cultures and the control were most pronounced during the early stages of the growth cycle and tapered off as the cultures matured. The tran...Continue Reading

References

Sep 12, 2002·Plant Physiology·A. Whittaker, F. C. Botha
Apr 6, 2004·FEBS Letters·Yuanhuai HanDon Grierson
Oct 14, 2004·European Journal of Biochemistry·Wolfgang E SchäferFrederik C Botha
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May 1, 1963·Plant Physiology·M D HatchK T Glasziou

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Citations

May 15, 2008·The Plant Journal : for Cell and Molecular Biology·Alison M Smith
Dec 4, 2008·Journal of Experimental Botany·A J McCormickM D Cramer
May 28, 2014·Biological Reviews of the Cambridge Philosophical Society·Douglas S Glazier
Aug 30, 2008·Biotechnology Journal·Stephanus J FerreiraJan-Hendrik Groenewald
Mar 16, 2012·Journal of Experimental Botany·Johann M Rohwer
Aug 21, 2012·Planta·Rolene BauerJens Kossmann
Oct 10, 2012·Plant Biotechnology Journal·John W PatrickRobert G Birch
Mar 9, 2021·Frontiers in Plant Science·Maria Juliana Calderan-RodriguesCamila Caldana

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