PMID: 7544757Sep 1, 1995Paper

Downregulation of phorbol 12-myristate 13-acetate-induced tumor necrosis factor-alpha and interleukin-1 beta production and gene expression in human monocytic cells by human alpha-fetoprotein

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
W Wang, E Alpert

Abstract

We previously identified a specific receptor of alpha-fetoprotein (AFP) on human monocytes. Although AFP alters many immune cell functions, the effect of AFP on monocyte cytokine production is unknown. Because tumor necrosis factor--alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) are important cytokines in immunoregulation, we investigated whether AFP could modulate TNF-alpha and IL-1 beta production in U937, a human monocytic cell line. Our results showed that U937 cells secreted TNF-alpha and IL-1 beta in response to either phorbyl 12-myristate 13-acetate (PMA) or IFN-gamma + LPS. In contrast, AFP significantly suppressed PMA-induced TNF-alpha and IL-1 beta production by U937 cells in a time and dose dependent fashion. Pretreatment of U937 cells with AFP resulted in maximal inhibition of PMA-stimulated TNF-alpha and IL-1 beta production by 58% and 67% respectively. AFP also inhibited interferon-gamma plus lipopolysaccharide (IFN-gamma + LPS)-induced TNF-alpha and IL-1 beta production. Furthermore, Northern blot analysis showed that AFP suppressed PMA-mediated TNF-alpha and IL-1 beta messenger RNA (mRNA) expression. PMA-induced prostaglandin E2 (PGE2) production by U937 cells was enhanced by AFP. Pretreatment with indomet...Continue Reading

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Citations

Feb 18, 1998·International Journal of Cancer. Journal International Du Cancer·X W Wang, B Xu
May 10, 2008·Journal of Gastroenterology and Hepatology·Noboru MitsuhashiMasaru Miyazaki
May 23, 2014·Frontiers in Immunology·Anne SchumacherAna Claudia Zenclussen
Nov 20, 1998·Lancet·H FarzadeganD Vlahov
Oct 1, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jenny W WangLawrence J Marnett

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