Downregulation of rheumatoid arthritis-related antigen RA-A47 (HSP47/colligin-2) in chondrocytic cell lines induces apoptosis and cell-surface expression of RA-A47 in association with CD9

Journal of Cellular Physiology
T HattoriM Takigawa

Abstract

Previously, we showed that gene expression of the rheumatoid arthritis-related antigen RA-A47, which is identical to human heat shock protein (HSP)47, was downregulated in chondrocytes by inflammatory cytokines such as TNFalpha. Associated with this phenomenon, RA-A47 appeared on the cell surface concomitant with upregulation of metabolic factors related to cartilage destruction. The upregulation of the metabolic factors could be achieved by downregulation of RA-A47 expression with ra-a47-specific anti-sense oligonucleotide. Here, we show that the enhanced surface expression of RA-A47 on a chondrocytic cell line, HCS-2/8 was also a direct result of RA-A47 downregulation by ra-a47 anti-sense oligonucleotide, independent of the cytokine effects. Moreover, cell-surface expression of CD9, a beta1 integrin-associated transmembrane protein that is involved in cell adhesion and cell motility events, was enhanced in the ra-a47 anti-sense oligonucleotide-treated cells. The CD9 was colocalized with RA-A47 on the cell surface, where it may have affected integrin signaling. Furthermore, Annexin-V binding to the cell surface and the level of a number of apoptosis-related genes including caspase-9 were increased after ra-a47 anti-sense oligo...Continue Reading

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Citations

Feb 28, 2007·Neuromuscular Disorders : NMD·Itsuro HiguchiMitsuhiro Osame
Dec 16, 2006·Trends in Molecular Medicine·Takashi Taguchi, M Shawkat Razzaque
Nov 7, 2006·Scandinavian Journal of Immunology·T MiletićM Dimitrijević
Nov 16, 2010·Biomedical Chromatography : BMC·Yu-Min JuangFuu-Jen Tsai
Mar 21, 2009·The Biochemical Journal·Eriko AoyamaMasaharu Takigawa
Mar 8, 2018·Journal of Thrombosis and Haemostasis : JTH·P SasikumarJ M Gibbins
Sep 6, 2017·Scientific Reports·Abhishek KumarChandan Goswami
Jul 7, 2012·The Journal of Pharmacology and Experimental Therapeutics·Nadine SchmidtAndrea Pautz
Sep 20, 2019·International Journal of Molecular Sciences·Eman A TahaTakanori Eguchi
Apr 23, 2020·International Journal of Molecular Sciences·Mitsuhiro HoshijimaMasaharu Takigawa

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