Downregulation of Sostdc1 in Testicular Sertoli Cells is Prerequisite for Onset of Robust Spermatogenesis at Puberty

Scientific Reports
Bhola Shankar PradhanSubeer S Majumdar

Abstract

An alarming decline in sperm count of men from several countries has become a major concern for the world community. Hormones act on testicular Sertoli cells (Sc) to regulate male fertility by governing the division and differentiation of germ cells (Gc). However, there is a limited knowledge about Sc specific gene(s) regulating the spermatogenic output of the testis. Sclerostin domain-containing 1 protein (Sostdc1) is a dual BMP/Wnt regulator is predominantly expressed in the Sc of infant testes which hardly show any sign of spermatogenesis. In order to investigate the role of Sostdc1 in spermatogenic regulation, we have generated transgenic (Tg) rats which induced persistent expression of Sostdc1 in mature Sc causing reduced sperm counts. Although Sc specific Sostdc1 did not affect the function of either Sc or Leydig cells (Lc) in the adult testis of Tg rat, we observed a selective augmentation of the BMP target genes via activated phospho smad 1/5/8 signaling in Gc leading to apoptosis. Here, for the first time, we have demonstrated that Sostdc1 is a negative regulator of spermatogenesis, and provided substantial evidence that down regulation of Sostdc1 during puberty is critically essential for quantitatively and qualitativ...Continue Reading

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Citations

Jul 21, 2020·Molecular Human Reproduction·Bhola Shankar PradhanSubeer S Majumdar
Jan 5, 2021·Molecular Human Reproduction·Ruizhi XuePeng Huang
Feb 6, 2021·Molecular Human Reproduction·Ruizhi XuePeng Huang
Aug 10, 2021·Frontiers in Endocrinology·Wasim ShahXiaohua Jiang

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Methods Mentioned

BETA
transgenic
PCR
biopsies
X ray

Software Mentioned

GraphPad Prism

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