Abstract
Substantial evidence indicates that the characteristic SS18-SSX fusion gene may play an important role in synovial sarcoma development and progression. For obtaining better insights into the genetic alterations and molecular mechanisms involved in synovial sarcomas and for developing novel therapeutic strategies for this disease, we first examined the efficiency of small interfering RNAs (siRNAs) targeting the SS18-SSX1 fusion gene in knocking down its expression in the human synovial sarcoma cell line HS-SY-II, and then evaluated the effects of downregulation of this gene on apoptosis, apoptosis-related gene expression, growth regulatory proteins, and the growth of tumor cells in vitro. We observed a marked decrease (by more than 87.6%) in SS18-SSX1 expression levels in cells transfected with a plasmid expressing hairpin siRNA for this gene, which was accompanied by (i) reduction in protein levels of cyclin D1 and cyclin A, (ii) reduction in antiapoptotic protein Bcl-2 and activation of caspase 3/apoptosis, and (iii) growth inhibition of HS-SY-II cells in vitro. Our results demonstrate that siRNA targeting of SS18-SSX1 may have therapeutic potential in the treatment of synovial sarcomas.
References
Aug 1, 1994·Nature Genetics·J ClarkC S Cooper
Jan 15, 1998·The New England Journal of Medicine·A KawaiM Ladanyi
Jun 8, 1999·Journal of the National Cancer Institute·B SkyttingO Larsson
May 16, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J J LewisM F Brennan
Dec 7, 2000·Genes, Chromosomes & Cancer·N R dos SantosA G van Kessel
Aug 29, 2001·Nature Structural Biology·P D Zamore
Oct 19, 2001·Oncogene·M Ladanyi
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 14, 2002·Cancer Genetics and Cytogenetics·Avery A Sandberg, Julia A Bridge
May 1, 2002·Nature Biotechnology·Makoto Miyagishi, Kazunari Taira
May 10, 2002·Nucleic Acids Research·Olivier Donzé, Didier Picard
Aug 13, 2002·Oncogene·Yuntao XieOlle Larsson
Dec 10, 2002·Current Opinion in Chemical Biology·Reuven Agami
Apr 12, 2003·Experimental Cell Research·Peter NagyThomas M Jovin
May 14, 2003·Molecular and Biochemical Parasitology·Jon P BoyleTimothy P Yoshino
Oct 25, 2003·Oncogene·Yuntao XieOlle Larsson
Jul 9, 2004·Nature·Carl D Novina, Phillip A Sharp
Jun 22, 2005·Clinical Imaging·Justin Q LyJoshua Packard
May 9, 2006·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Baocun SunXishan Hao
Citations
Mar 8, 2012·Molekuliarnaia biologiia·T V KekeevaG A Frank
May 30, 2013·Clinical Orthopaedics and Related Research·Emily E Carmody SoniJeffrey A Toretsky
Jan 19, 2013·Medical Oncology·Monica CantileGerardo Botti
May 3, 2012·Sarcoma·Joanna PrzybylJanusz A Siedlecki
Jun 24, 2016·Annals of Surgical Oncology·Myrella VlenterieWinette T A van der Graaf
Mar 4, 2014·PloS One·Yuki KamijhoDaisuke Watanabe
Sep 12, 2013·British Journal of Cancer·T RenY Sun
Aug 31, 2016·Oncotarget·Changliang PengChunzheng Gao
Mar 14, 2018·International Journal of Oncology·Changliang PengChunzheng Gao
May 25, 2020·Journal of Cancer Research and Clinical Oncology·Ning XuXin Gou
Jan 27, 2019·Oncotarget·Midori IshiiTsuyoshi Saito
Apr 23, 2020·Sarcoma·Kirill I KirsanovMarianna G Yakubovskaya
Nov 5, 2020·Epigenetics & Chromatin·Sarah M Innis, Birgit Cabot
May 25, 2021·Molecular & Cellular Oncology·Christina Cooley, Le Su